Effect of dietary enrichment with eicosapentaenoic and docosahexaenoic acids on in vitro neutrophil and monocyte leukotriene generation and neutrophil function
TH Lee, RL Hoover, JD Williams, RI Sperling, J Ravalese, BW Spur, DR Robinson, EJ Corey, RA Lewis, and KF Austen
The effects of dietary fish-oil fatty acids on the function of the 5-lipoxygenase pathway of peripheral-blood polymorphonuclear leukocytes and monocytes were determined in seven normal subjects who supplemented their usual diet for six weeks with daily doses of triglycerides containing 3.2 g of eicosapentaenoic acid and 2.2 g of docosahexaenoic acid. The diet increased the eicosapentaenoic acid content in neutrophils and monocytes more than sevenfold, without changing the quantities of arachidonic acid and docosahexaenoic acid. When the neutrophils were activated, the release of [3H]arachidonic acid and its labeled metabolites was reduced by a mean of 37 per cent, and the maximum generation of three products of the 5-lipoxygenase pathway was reduced by more than 48 per cent. The ionophore-induced release of [3H]arachidonic acid and its labeled metabolites from monocytes in monolayers was reduced by a mean of 39 per cent, and the generation of leukotriene B4 by 58 per cent. The adherence of neutrophils to bovine endothelial-cell monolayers pretreated with leukotriene B4 was inhibited completely, and their average chemotactic response to leukotriene B4 was inhibited by 70 per cent, as compared with values determined before the diet was begun and six weeks after its discontinuation. We conclude that diets enriched with fish-oil-derived fatty acids may have antiinflammatory effects by inhibiting the 5-lipoxygenase pathway in neutrophils and monocytes and inhibiting the leukotriene B4-mediated functions of neutrophils.
This article has been cited by other articles:
Eneli, I U, Skybo, T, Camargo, C A Jr
(2008). Weight loss and asthma: a systematic review. Thorax
63: 671-676
[Abstract][Full Text]
Farran, T. B., Reinhardt, C. D., Blasi, D. A., Minton, J. E., Elsasser, T. H., Higgins, J. J., Drouillard, J. S.
(2008). Source of dietary lipid may modify the immune response in stressed feeder cattle. J ANIM SCI
86: 1382-1394
[Abstract][Full Text]
Wang, X., Li, W., Li, N., Li, J.
(2008). {omega}-3 Fatty Acids-Supplemented Parenteral Nutrition Decreases Hyperinflammatory Response and Attenuates Systemic Disease Sequelae in Severe Acute Pancreatitis: A Randomized and Controlled Study. JPEN J Parenter Enteral Nutr
32: 236-241
[Abstract][Full Text]
Saifullah, A., Watkins, B. A., Saha, C., Li, Y., Moe, S. M., Friedman, A. N.
(2007). Oral fish oil supplementation raises blood omega-3 levels and lowers C-reactive protein in haemodialysis patients a pilot study. Nephrol Dial Transplant
22: 3561-3567
[Abstract][Full Text]
Koto, T., Nagai, N., Mochimaru, H., Kurihara, T., Izumi-Nagai, K., Satofuka, S., Shinoda, H., Noda, K., Ozawa, Y., Inoue, M., Tsubota, K., Oike, Y., Ishida, S.
(2007). Eicosapentaenoic Acid Is Anti-Inflammatory in Preventing Choroidal Neovascularization in Mice. IOVS
48: 4328-4334
[Abstract][Full Text]
Chen, W., Jump, D. B., Esselman, W. J., Busik, J. V.
(2007). Inhibition of Cytokine Signaling in Human Retinal Endothelial Cells through Modification of Caveolae/Lipid Rafts by Docosahexaenoic Acid. IOVS
48: 18-26
[Abstract][Full Text]
Pastor, N., Soler, B., Mitmesser, S. H., Ferguson, P., Lifschitz, C.
(2006). Infants Fed Docosahexaenoic Acid- and Arachidonic Acid-Supplemented Formula Have Decreased Incidence of Bronchiolitis/Bronchitis the First Year of Life. CLIN PEDIATR
45: 850-855
[Abstract]
Aeberli, I., Molinari, L., Spinas, G., Lehmann, R., l'Allemand, D., Zimmermann, M. B
(2006). Dietary intakes of fat and antioxidant vitamins are predictors of subclinical inflammation in overweight Swiss children.. Am. J. Clin. Nutr.
84: 748-755
[Abstract][Full Text]
Lee, S., Gura, K. M., Kim, S., Arsenault, D. A., Bistrian, B. R., Puder, M.
(2006). Current Clinical Applications of {Omega}-6 and {Omega}-3 Fatty Acids. Nutr Clin Pract
21: 323-341
[Abstract][Full Text]
Calder, P. C
(2006). n-3 Polyunsaturated fatty acids, inflammation, and inflammatory diseases. Am. J. Clin. Nutr.
83: S1505-1519S
[Abstract][Full Text]
Friedman, A., Moe, S.
(2006). Review of the Effects of Omega-3 Supplementation in Dialysis Patients. CJASN
1: 182-192
[Abstract][Full Text]
Iso, H., Kobayashi, M., Ishihara, J., Sasaki, S., Okada, K., Kita, Y., Kokubo, Y., Tsugane, S., for the JPHC Study Group,
(2006). Intake of Fish and n3 Fatty Acids and Risk of Coronary Heart Disease Among Japanese: The Japan Public Health Center-Based (JPHC) Study Cohort I. Circulation
113: 195-202
[Abstract][Full Text]
Mickleborough, T. D., Lindley, M. R., Ionescu, A. A., Fly, A. D.
(2006). Protective Effect of Fish Oil Supplementation on Exercise-Induced Bronchoconstriction in Asthma. Chest
129: 39-49
[Abstract][Full Text]
Phinney, S. D
(2005). Fatty acids, inflammation, and the metabolic syndrome. Am. J. Clin. Nutr.
82: 1151-1152
[Full Text]
Chen, W., Esselman, W. J., Jump, D. B., Busik, J. V.
(2005). Anti-inflammatory Effect of Docosahexaenoic Acid on Cytokine-Induced Adhesion Molecule Expression in Human Retinal Vascular Endothelial Cells. IOVS
46: 4342-4347
[Abstract][Full Text]
Jacobson, K., Mundra, H., Innis, S. M.
(2005). Intestinal responsiveness to experimental colitis in young rats is altered by maternal diet. Am. J. Physiol. Gastrointest. Liver Physiol.
289: G13-G20
[Abstract][Full Text]
Dwyer, J. H., Allayee, H., Dwyer, K. M., Fan, J., Wu, H., Mar, R., Lusis, A. J., Mehrabian, M.
(2004). Arachidonate 5-Lipoxygenase Promoter Genotype, Dietary Arachidonic Acid, and Atherosclerosis. NEJM
350: 29-37
[Abstract][Full Text]
Sadeh, J., Israel, E.
(2003). Airway Narrowing in Athletes: A Different Kettle of Fish?. Am. J. Respir. Crit. Care Med.
168: 1146-1147
[Full Text]
Mickleborough, T. D., Murray, R. L., Ionescu, A. A., Lindley, M. R.
(2003). Fish Oil Supplementation Reduces Severity of Exercise-induced Bronchoconstriction in Elite Athletes. Am. J. Respir. Crit. Care Med.
168: 1181-1189
[Abstract][Full Text]
OSTERUD, B., BJORKLID, E.
(2003). Role of Monocytes in Atherogenesis. Physiol. Rev.
83: 1069-1112
[Abstract][Full Text]
Lee, P., Prasad, K.
(2003). Effects of Flaxseed Oil on Serum Lipids and Atherosclerosis in Hypercholesterolemic Rabbits. J CARDIOVASC PHARMACOL THER
8: 227-235
[Abstract]
Kew, S., Banerjee, T., Minihane, A. M, Finnegan, Y. E, Williams, C. M, Calder, P. C
(2003). Relation between the fatty acid composition of peripheral blood mononuclear cells and measures of immune cell function in healthy, free-living subjects aged 25-72 y. Am. J. Clin. Nutr.
77: 1278-1286
[Abstract][Full Text]
Terada, S., Takizawa, M., Yamamoto, S., Ezaki, O., Itakura, H., Akagawa, K. S.
(2002). Eicosapentaenoic acid inhibits CSF-induced human monocyte survival and maturation into macrophage through the stimulation of H2O2 production. J. Leukoc. Biol.
71: 981-986
[Abstract][Full Text]
Hernandez, D., Guerra, R., Milena, A., Torres, A., Garcia, S., Garcia, C., Abreu, P., Gonzalez, A., Gomez, M. A., Rufino, M., Gonzalez-Posada, J., Lorenzo, V., Salido, E.
(2002). Dietary fish oil does not influence acute rejection rate and graft survival after renal transplantation: a randomized placebo-controlled study. Nephrol Dial Transplant
17: 897-904
[Abstract][Full Text]
Jeejeebhoy, K. N.
(2002). Clinical nutrition: 6. Management of nutritional problems of patients with Crohn's disease. CMAJ
166: 913-918
[Full Text]
Schmitz, P. G., Antony, K. A.
(2002). {omega}-3 fatty acids in ESRD: should patients with ESRD eat more fish?. Nephrol Dial Transplant
17: 11-14
[Full Text]
Schmitz, P. G., McCloud, L. K., Reikes, S. T., Leonard, C. L., Gellens, M. E.
(2002). Prophylaxis of Hemodialysis Graft Thrombosis with Fish Oil: Double-Blind, Randomized, Prospective Trial. J. Am. Soc. Nephrol.
13: 184-190
[Abstract][Full Text]
Nieto, N., Torres, M. I., Rios, A., Gil, A.
(2002). Dietary Polyunsaturated Fatty Acids Improve Histological and Biochemical Alterations in Rats with Experimental Ulcerative Colitis. J. Nutr.
132: 11-19
[Abstract][Full Text]
Allen, K. G.D., Harris, M. A.
(2001). The Role of n-3 Fatty Acids in Gestation and Parturition. Exp. Biol. Med.
226: 498-506
[Abstract][Full Text]
Simopoulos, A.P.
(2001). Evolutionary aspects of diet, essential fatty acids and cardiovascular disease. Eur Heart J Suppl
3: D8-D21
[Abstract]
De Caterina, R., Basta, G.
(2001). n-3 Fatty acids and the inflammatory response -- biological background. Eur Heart J Suppl
3: D42-D49
[Abstract]
Boelsma, E., Hendriks, H. F., Roza, L.
(2001). Nutritional skin care: health effects of micronutrients and fatty acids. Am. J. Clin. Nutr.
73: 853-864
[Abstract][Full Text]
Iso, H., Rexrode, K. M., Stampfer, M. J., Manson, J. E., Colditz, G. A., Speizer, F. E., Hennekens, C. H., Willett, W. C.
(2001). Intake of Fish and Omega-3 Fatty Acids and Risk of Stroke in Women. JAMA
285: 304-312
[Abstract][Full Text]
Granot, E., Golan, D., Berry, E. M
(2000). Breast-fed and formula-fed infants do not differ in immunocompetent cell cytokine production despite differences in cell membrane fatty acid composition. Am. J. Clin. Nutr.
72: 1202-1205
[Abstract][Full Text]
Zhang, J., Temme, E. H., Kesteloot, H.
(2000). Fish consumption is inversely associated with male lung cancer mortality in countries with high levels of cigarette smoking or animal fat consumption. Int J Epidemiol
29: 615-621
[Abstract][Full Text]
Curtis, C. L., Hughes, C. E., Flannery, C. R., Little, C. B., Harwood, J. L., Caterson, B.
(2000). n-3 Fatty Acids Specifically Modulate Catabolic Factors Involved in Articular Cartilage Degradation. J. Biol. Chem.
275: 721-724
[Abstract][Full Text]
Eritsland, J.
(2000). Safety considerations of polyunsaturated fatty acids. Am. J. Clin. Nutr.
71: 197S-201S
[Abstract][Full Text]
Belluzzi, A., Boschi, S., Brignola, C., Munarini, A., Cariani, G., Miglio, F.
(2000). Polyunsaturated fatty acids and inflammatory bowel disease1. Am. J. Clin. Nutr.
71: 339S-342S
[Abstract][Full Text]
Kremer, J. M
(2000). n-3 Fatty acid supplements in rheumatoid arthritis1. Am. J. Clin. Nutr.
71: 349S-351S
[Abstract][Full Text]
Schwartz, J.
(2000). Role of polyunsaturated fatty acids in lung disease 1. Am. J. Clin. Nutr.
71: 393S-396S
[Abstract][Full Text]
Burns, C. P., Halabi, S., Clamon, G. H., Hars, V., Wagner, B. A., Hohl, R. J., Lester, E., Kirshner, J. J., Vinciguerra, V., Paskett, E.
(1999). Phase I Clinical Study of Fish Oil Fatty Acid Capsules for Patients with Cancer Cachexia: Cancer and Leukemia Group B Study 9473. Clin. Cancer Res.
5: 3942-3947
[Abstract][Full Text]
SHAHAR, E., BOLAND, L. L., FOLSOM, A. R., TOCKMAN, M. S., MCGOVERN, P. G., ECKFELDT, J. H.
(1999). Docosahexaenoic Acid and Smoking-Related Chronic Obstructive Pulmonary Disease. Am. J. Respir. Crit. Care Med.
159: 1780-1785
[Abstract][Full Text]
Cadroy, Y., Dupouy, D., Boneu, B.
(1998). Arachidonic Acid Enhances the Tissue Factor Expression of Mononuclear Cells by the Cyclo-Oxygenase-1 Pathway: Beneficial Effect of n-3 Fatty Acids. J. Immunol.
160: 6145-6150
[Abstract][Full Text]
Rayon, J. I., Carver, J. D., Wyble, L. E., Wiener, D., Dickey, S. S., Benford, V. J., Chen, L. T., Lim, D. V.
(1997). The Fatty Acid Composition of Maternal Diet Affects Lung Prostaglandin E2 Levels and Survival from Group B Streptococcal Sepsis in Neonatal Rat Pups. J. Nutr.
127: 1989-1992
[Abstract][Full Text]
Johnson, M. M., Swan, D. D., Surette, M. E., Stegner, J., Chilton, T., Fonteh, A. N., Chilton, F. H.
(1997). Dietary Supplementation with gamma -Linolenic Acid Alters Fatty Acid Content and Eicosanoid Production in Healthy Humans. J. Nutr.
127: 1435-1444
[Abstract][Full Text]
Sen, C. K., Atalay, M., Agren, J., Laaksonen, D. E., Roy, S., Hanninen, O.
(1997). Fish oil and vitamin E supplementation in oxidative stress at rest and after physical exercise. J. Appl. Physiol.
83: 189-195
[Abstract][Full Text]
Belluzzi, A., Brignola, C., Campieri, M., Pera, A., Boschi, S., Miglioli, M.
(1996). Effect of an Enteric-Coated Fish-Oil Preparation on Relapses in Crohn's Disease. NEJM
334: 1557-1560
[Abstract][Full Text]
Hyltander, A., Sandstrom, R., Lundholm, K.
(1995). Invited Review: Metabolic Effects of Structured Triglycerides in Humans. Nutr Clin Pract
10: 91-97
[Abstract]
Wallace, J. M. W., Turley, E., Gilmore, W. S., Strain, J. J.
(1995). Dietary Fish Oil Supplementation Alters Leukocyte Function and Cytokine Production in Healthy Women. Arterioscler. Thromb. Vasc. Bio.
15: 185-189
[Abstract][Full Text]
Salachas, A., Papadopoulos, C., Sakadamis, G., Styliadis, J., Voudris, V., Oakley, D., Saynor, R.
(1994). Effects of a Low-Dose Fish Oil Concentrate on Angina, Exercise Tolerance Time, Serum Triglycerides, and Platelet Function. ANGIOLOGY
45: 1023-1031
[Abstract]
Shahar, E., Folsom, A. R., Melnick, S. L., Tockman, M. S., Comstock, G. W., Gennaro, V., Higgins, M. W., Sorlie, P. D., Ko, W.-J., Szklo, M., The Atherosclerosis Risk in Communities Study Inve,
(1994). Dietary n-3 Polyunsaturated Fatty Acids and Smoking-Related Chronic Obstructive Pulmonary Disease. NEJM
331: 228-233
[Abstract][Full Text]
van der Heide, J. J. H., Bilo, H., Donker, J.M., Wilmink, J. M., Tegzess, A. M.
(1993). Effect of Dietary Fish Oil on Renal Function and Rejection in Cyclosporine-Treated Recipients of Renal Transplants. NEJM
329: 769-773
[Abstract][Full Text]
Soyland, E., Funk, J., Rajka, G., Sandberg, M., Thune, P., Rustad, L., Helland, S., Middelfart, K., Odu, S., Falk, E. S., Solvoll, K., Bjorneboe, G.-E. A., Drevon, C. A.
(1993). Effect of Dietary Supplementation with Very-Long-Chain n-3 Fatty Acids in Patients with Psoriasis. NEJM
328: 1812-1816
[Abstract][Full Text]
Gottschlich, M. M.
(1992). Invited Review: Selection of Optimal Lipid Sources in Enteral and Parenteral Nutrition. Nutr Clin Pract
7: 152-165
[Abstract]
Bowles, M. H., Klonis, D., Plavac, T. G., Gonzales, B., Francisco, D. A., Roberts, R. W., Boxberger, G. R., Poliner, L. R., Galichia, J. P.
(1991). EPA in the Prevention of Restenosis Post PTCA. ANGIOLOGY
42: 187-194
[Abstract]
Franz, M., Kulkarni, K.
(1985). Nutrition Update. The Diabetes Educator
11: 58-58
Previous
