Although blood eosinophilia is commonly present in atopic dermatitis, accumulation of tissue eosinophils is not prominent. To determine whether eosinophil degranulation occurs in lesions of atopic dermatitis, we analyzed tissues by immunofluorescence for the presence of the eosinophil-granule major basic protein. Twenty biopsy specimens from 18 patients with atopic dermatitis were studied, and all showed major basic protein staining outside eosinophils. In 18 specimens, the staining was fibrillar, was located in the upper half of the dermis, and was similar to the distribution of elastic fibers. Twelve specimens with fibrillar staining also showed major basic protein staining in the form of extracellular granules. One specimen from unaffected skin showed minimal faint, fine, fluorescing fibrils, but there was marked deposition of the protein in affected skin. The fibrillar pattern of major basic protein staining in atopic dermatitis was very similar to that seen in lichenified lesions of untreated onchocerciasis. These results suggest that eosinophils commonly release granule proteins in the dermis and that assessment of eosinophil involvement in disease cannot be based simply on numbers of eosinophils in tissue.
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162: 4960-4965
[Abstract][Full Text]
Bartemes, K. R., McKinney, S., Gleich, G. J., Kita, H.
(1999). Endogenous Platelet-Activating Factor Is Critically Involved in Effector Functions of Eosinophils Stimulated with IL-5 or IgG. J. Immunol.
162: 2982-2989
[Abstract][Full Text]
Boguniewicz, M., Leung, D. Y. M.
(1998). Atopic Dermatitis: A Question of Balance. Arch Dermatol
134: 870-871
[Full Text]
Heiligenhaus, A., Schaller, J., Mauß, S., Engelbrecht, S., Dutt, J. E, Foster, C S., Steuhl, K.-P.
(1998). Eosinophil granule proteins expressed in ocular cicatricial pemphigoid. Br J Ophthalmol
82: 312-317
[Abstract][Full Text]
Mochizuki, M., Bartels, J., Mallet, A. I., Christophers, E., Schroder, J.-M.
(1998). IL-4 Induces Eotaxin: A Possible Mechanism of Selective Eosinophil Recruitment in Helminth Infection and Atopy. J. Immunol.
160: 60-68
[Abstract][Full Text]
Okada, S., Kita, H., George, T. J., Gleich, G. J., Leiferman, K. M.
(1997). Migration of Eosinophils through Basement Membrane Components In Vitro: Role of Matrix Metalloproteinase-9. Am. J. Respir. Cell Mol. Bio.
17: 519-528
[Abstract][Full Text]
LAMBLIN, C., GOSSET, P., TILLIE-LEBLOND, I., SAULNIER, F., MARQUETTE, C.-H., WALLAERT, B., TONNEL, A. B.
(1997). Bronchial Neutrophilia in Patients with Noninfectious Status Asthmaticus. Am. J. Respir. Crit. Care Med.
157: 394-402
[Abstract][Full Text]
Hanifin, J. M.
(1996). Assembling the Puzzle Pieces in Atopic Inflammation. Arch Dermatol
132: 1230-1232
[Abstract]
Schwenk, U., Schröder, J.-M.
(1995). 5-Oxo-eicosanoids Are Potent Eosinophil Chemotactic Factors. J. Biol. Chem.
270: 15029-15036
[Abstract][Full Text]
Perez, G. L., Peters, M. S., Reda, A. M., Butterfield, J. H., Peterson, E. A., Leiferman, K. M.
(1993). Mast Cells, Neutrophils, and Eosinophils in Prurigo Nodularis. Arch Dermatol
129: 861-865
[Abstract]
Horan, R. F., Schneider, L. C., Sheffer, A. L.
(1992). Allergic Skin Disorders and Mastocytosis. JAMA
268: 2858-2868
[Abstract]
Trocme, S. D., Bartley, G. B., Campbell, R. J., Gleich, G. J., Leiferman, K. M.
(1991). Eosinophil and Neutrophil Degranulation in Ophthalmic Lesions of Wegener's Granulomatosis. Arch Ophthalmol
109: 1585-1589
[Abstract]
Wegner, C., Gundel, R., Reilly, P, Haynes, N, Letts, L., Rothlein, R
(1990). Intercellular adhesion molecule-1 (ICAM-1) in the pathogenesis of asthma. Science
247: 456-459
[Abstract]
Clark, R. A. F.
(1989). Cell-Mediated and IgE-Mediated Immune Responses in Atopic Dermatitis. Arch Dermatol
125: 413-416
[Abstract]
Norris, P. G., Murphy, G. M., Hawk, J. L. M., Winkelmann, R. K.
(1988). A Histological Study of the Evolution of Solar Urticaria. Arch Dermatol
124: 80-83
[Abstract]
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