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Previous Volume 339:223-228 July 23, 1998 Number 4 Next

	Full Text PDF Editorial by Lindblom, A. Letters Add to Personal Archive Add to Citation Manager Notify a Friend E-mail When Cited PubMed Citation

ABSTRACT

Background Standard treatment of colorectal cancer includes adjuvant chemotherapy for patients with stage III disease (defined by the presence of lymph-node metastases), but not for patients with stage II tumors (who have no lymph-node metastases). However, 20 percent of patients with stage II tumors die of recurrent disease. We investigated whether the detection of micrometastases can be used to identify patients with stage II disease who are at high risk for recurrence.

Methods We analyzed 192 lymph nodes from 26 consecutive patients with stage II colorectal cancer, using a carcinoembryonic antigen–specific nested reverse-transcriptase polymerase chain reaction. Five-year follow-up information was obtained on all patients. Observed and adjusted survival rates were assessed in the patients with and the patients without micrometastases.

Results Micrometastases were detected in one or more lymph nodes from 14 of 26 patients (54 percent). The adjusted five-year survival rate (for which only cancer-related deaths were considered) was 50 percent in this group, whereas in the 12 patients without micrometastases, the survival rate was 91 percent (P=0.02 by the log-rank test). The observed five-year survival rates were 36 percent and 75 percent, respectively (P=0.03). The groups were similar with respect to age, sex, tumor side (location in relation to the flexura lienalis), degree of tumor differentiation (grade), and diameter of the primary tumor.

Conclusions Molecular detection of micrometastases is a prognostic tool in stage II colorectal cancer.

Source Information

From the Department of Surgery (G.-J.L., C.J.H.V., R.A.E.M.T.), the Department of Pathology (A.-M.C.-J., J.H.J.M.K., C.J.C.), and the Department of Medical Statistics (J.H.), Leiden University Medical Center, Leiden, the Netherlands.

Address reprint requests to Dr. Tollenaar at the Department of Surgery K6R, Leiden University Medical Center, P.O. Box 9600, 2300 RC, Leiden, the Netherlands.

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N Engl J Med 1998; 339:1642-1644, Nov 26, 1998. Correspondence

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• Raja, S., Luketich, J. D., Kelly, L. A., Gooding, W. E., Finkelstein, S. D., Godfrey, T. E. (2002). Rapid, quantitative reverse transcriptase-polymerase chain reaction: Application to intraoperative molecular detection of occult metastases in esophageal cancer. J. Thorac. Cardiovasc. Surg. 123: 475-483 [Abstract] [Full Text]  
• D'Cunha, J., Corfits, A. L., Herndon, J. E. II, Kern, J. A., Kohman, L. J., Patterson, G. A., Kratzke, R. A., Maddaus, M. A. (2002). Molecular staging of lung cancer: Real-time polymerase chain reaction estimation of lymph node micrometastatic tumor cell burden in stage I non-small cell lung cancer--preliminary results of cancer and leukemia group B trial 9761. J. Thorac. Cardiovasc. Surg. 123: 484-491 [Abstract] [Full Text]  
• Noura, S., Yamamoto, H., Miyake, Y., no Kim, B., Takayama, O., Seshimo, I., Ikenaga, M., Ikeda, M., Sekimoto, M., Matsuura, N., Monden, M. (2002). Immunohistochemical Assessment of Localization and Frequency of Micrometastases in Lymph Nodes of Colorectal Cancer. Clin. Cancer Res. 8: 759-767 [Abstract] [Full Text]  
• Rosenberg, R., Hoos, A., Mueller, J., Baier, P., Stricker, D., Werner, M., Nekarda, H., Siewert, J.-R. (2002). Prognostic Significance of Cytokeratin-20 Reverse Transcriptase Polymerase Chain Reaction in Lymph Nodes of Node-Negative Colorectal Cancer Patients. JCO 20: 1049-1055 [Abstract] [Full Text]  
• Godfrey, T. E., Raja, S., Finkelstein, S. D., Gooding, W. E., Kelly, L. A., Luketich, J. D. (2001). Prognostic Value of Quantitative Reverse Transcription-Polymerase Chain Reaction in Lymph Node-negative Esophageal Cancer Patients. Clin. Cancer Res. 7: 4041-4048 [Abstract] [Full Text]  
• Zealley, I. A., Skehan, S. J., Rawlinson, J., Coates, G., Nahmias, C., Somers, S. (2001). Selection of Patients for Resection of Hepatic Metastases: Improved Detection of Extrahepatic Disease with FDG PET. RadioGraphics 21: S55-69 [Abstract] [Full Text]  
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• Miyake, Y., Yamamoto, H., Fujiwara, Y., Ohue, M., Sugita, Y., Tomita, N., Sekimoto, M., Matsuura, N., Shiozaki, H., Monden, M. (2001). Extensive Micrometastases to Lymph Nodes as a Marker for Rapid Recurrence of Colorectal Cancer: A Study of Lymphatic Mapping. Clin. Cancer Res. 7: 1350-1357 [Abstract] [Full Text]  
• Wood, T. F., Saha, S., Morton, D. L., Tsioulias, G. J., Rangel, D., Hutchinson, W. Jr., Foshag, L. J., Bilchik, A. J. (2001). Validation of Lymphatic Mapping in Colorectal Cancer: In Vivo, Ex Vivo, and Laparoscopic Techniques. Ann. Surg. Oncol. 8: 150-157 [Abstract] [Full Text]  
• Morgagni, P., Saragoni, L., Folli, S., Gaudio, M., Scarpi, E., Bazzocchi, F., Marra, G. A., Vio, A. (2001). Lymph Node Micrometastases in Patients With Early Gastric Cancer: Experience With 139 Patients. Ann. Surg. Oncol. 8: 170-174 [Abstract] [Full Text]  
• Bilchik, A. J., Saha, S., Wiese, D., Stonecypher, J. A., Wood, T. F., Sostrin, S., Turner, R. R., Wang, H.-J., Morton, D. L., Hoon, D. S.B. (2001). Molecular Staging of Early Colon Cancer on the Basis of Sentinel Node Analysis: A Multicenter Phase II Trial. JCO 19: 1128-1136 [Abstract] [Full Text]  
• Moesta, K. T., Ebert, B., Handke, T., Nolte, D., Nowak, C., Haensch, W. E., Pandey, R. K., Dougherty, T. J., Rinneberg, H., Schlag, P. M. (2001). Protoporphyrin IX Occurs Naturally in Colorectal Cancers and Their Metastases. Cancer Res. 61: 991-999 [Abstract] [Full Text]  
• Clayton, S. J., Scott, F. M., Walker, J., Callaghan, K., Haque, K., Liloglou, T., Xinarianos, G., Shawcross, S., Ceuppens, P., Field, J. K., Fox, J. C. (2000). K-ras Point Mutation Detection in Lung Cancer: Comparison of Two Approaches to Somatic Mutation Detection Using ARMS Allele-specific Amplification. Clin. Chem. 46: 1929-1938 [Abstract] [Full Text]  
• Masuda, N., Tamaki, Y., Sakita, I., Ooka, M., Ohnishi, T., Kadota, M., Aritake, N., Okubo, K., Monden, M. (2000). Clinical Significance of Micrometastases in Axillary Lymph Nodes Assessed by Reverse Transcription-Polymerase Chain Reaction in Breast Cancer Patients. Clin. Cancer Res. 6: 4176-4185 [Abstract] [Full Text]  
• van Houten, V. M. M., Tabor, M. P., van den Brekel, M. W. M., Denkers, F., Wishaupt, R. G. A., Kummer, J. A., Snow, G. B., Brakenhoff, R. H. (2000). Molecular Assays for the Diagnosis of Minimal Residual Head-and-Neck Cancer: Methods, Reliability, Pitfalls, and Solutions. Clin. Cancer Res. 6: 3803-3816 [Abstract] [Full Text]  
• Cascinu, S., Staccioli, M. P., Gasparini, G., Giordani, P., Catalano, V., Ghiselli, R., Rossi, C., Baldelli, A. M., Graziano, F., Saba, V., Muretto, P., Catalano, G. (2000). Expression of Vascular Endothelial Growth Factor Can Predict Event-free Survival in Stage II Colon Cancer. Clin. Cancer Res. 6: 2803-2807 [Abstract] [Full Text]  
• Takemasa, I., Yamamoto, H., Sekimoto, M., Ohue, M., Noura, S., Miyake, Y., Matsumoto, T., Aihara, T., Tomita, N., Tamaki, Y., Sakita, I., Kikkawa, N., Matsuura, N., Shiozaki, H., Monden, M. (2000). Overexpression of CDC25B Phosphatase as a Novel Marker of Poor Prognosis of Human Colorectal Carcinoma. Cancer Res. 60: 3043-3050 [Abstract] [Full Text]  
• Okami, J., Dohno, K., Sakon, M., Iwao, K., Yamada, T., Yamamoto, H., Fujiwara, Y., Nagano, H., Umeshita, K., Matsuura, N., Nakamori, S., Monden, M. (2000). Genetic Detection for Micrometastasis in Lymph Node of Biliary Tract Carcinoma. Clin. Cancer Res. 6: 2326-2332 [Abstract] [Full Text]  
• Lindblom, A., Liljegren, A. (2000). Regular review: Tumour markers in malignancies. BMJ 320: 424-427 [Full Text]  
• Guan, R. J., Ford, H. L., Fu, Y., Li, Y., Shaw, L. M., Pardee, A. B. (2000). Drg-1 as a Differentiation-related, Putative Metastatic Suppressor Gene in Human Colon Cancer. Cancer Res. 60: 749-755 [Abstract] [Full Text]  
• Cagir, B., Gelmann, A., Park, J., Fava, T., Tankelevitch, A., Bittner, E. W., Weaver, E. J., Palazzo, J. P., Weinberg, D., Fry, R. D., Waldman, S. A. (1999). Guanylyl Cyclase C Messenger RNA Is a Biomarker for Recurrent Stage II Colorectal Cancer. ANN INTERN MED 131: 805-812 [Abstract] [Full Text]  
• Sanchez-Cespedes, M., Esteller, M., Hibi, K., Cope, F. O., Westra, W. H., Piantadosi, S., Herman, J. G., Jen, J., Sidransky, D. (1999). Molecular Detection of Neoplastic Cells in Lymph Nodes of Metastatic Colorectal Cancer Patients Predicts Recurrence. Clin. Cancer Res. 5: 2450-2454 [Abstract] [Full Text]  
• Ghossein, R. A., Bhattacharya, S., Rosai, J. (1999). Molecular Detection of Micrometastases and Circulating Tumor Cells in Solid Tumors. Clin. Cancer Res. 5: 1950-1960 [Abstract] [Full Text]  
• Pantel, K., Cote, R. J., Fodstad, O. (1999). Detection and Clinical Importance of Micrometastatic Disease. JNCI J Natl Cancer Inst 91: 1113-1124 [Abstract] [Full Text]  
• Weitz, J., Kienle, P., Magener, A., Koch, M., Schrodel, A., Willeke, F., Autschbach, F., Lacroix, J., Lehnert, T., Herfarth, C., Doeberitz, M. v. K. (1999). Detection of Disseminated Colorectal Cancer Cells in Lymph Nodes, Blood and Bone Marrow. Clin. Cancer Res. 5: 1830-1836 [Abstract] [Full Text]  
• Merrie, A. E.H., Yun, K., McCall, J. L., Ghossein, R. A., Bostick, P. J., Hoon, D. S.B., Cote, R. J., Liefers, G.-J., Cleton-Jansen, A.-M., Tollenaar, R. A.E.M. (1998). Detection of Carcinoembryonic Antigen Messenger RNA in Lymph Nodes from Patients with Colorectal Cancer. NEJM 339: 1642-1644 [Full Text]  
• Lindblom, A. (1998). Improved Tumor Staging in Colorectal Cancer. NEJM 339: 264-265 [Full Text]