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Previous Volume 347:488-496 August 15, 2002 Number 7 Next

	Full Text PDF PDA Full Text PowerPoint Slide Set Editorial by Fishman, J. A. Letters Add to Personal Archive Add to Citation Manager Notify a Friend E-mail When Cited PubMed Citation

ABSTRACT

Background Nephropathy associated with the polyomavirus type BK (BKV) nephropathy has emerged as a cause of allograft failure linked to immunosuppressive regimens containing tacrolimus or mycophenolate mofetil. The presence of viral inclusions, known as "decoy cells," in urine and the presence of BKV DNA in plasma have been proposed as markers for the replication of BKV and associated nephropathy, but data from prospective studies have been lacking.

Methods In a prospective, single-center study, we followed 78 renal-transplant recipients who were receiving immunosuppressive therapy that included tacrolimus (37 patients) or mycophenolate mofetil (41 patients). Urine was tested for the presence of decoy cells at routine visits. BKV DNA was measured 3, 6, and 12 months after transplantation and whenever decoy cells were detected. The viral load in plasma was quantified with the use of a real-time polymerase-chain-reaction method. Renal biopsy was performed if allograft function deteriorated.

Results Twenty-three patients had decoy-cell shedding a median of 16 weeks after transplantation (range, 2 to 69), 10 patients had BKV viremia at a median of 23 weeks (range, 4 to 73), and 5 had BKV nephropathy at a median of 28 weeks (range, 8 to 86). Kaplan–Meier estimates of the probability of decoy-cell shedding, viremia, and nephropathy were 30 percent (95 percent confidence interval, 20 to 40 percent), 13 percent (95 percent confidence interval, 5 to 21 percent), and 8 percent (95 percent confidence interval, 1 to 15 percent), respectively. Antirejection treatment, particularly with corticosteroids, was associated with BKV replication and nephropathy. The viral load in plasma was higher in patients with BKV nephropathy than in those without nephropathy (P<0.001 by the Mann–Whitney test). BKV antibodies were detected in 77 percent of the 78 patients before transplantation, including 4 of 5 with BKV nephropathy.

Conclusions BKV nephropathy in renal-transplant recipients represents a secondary infection associated with rejection and its treatment in most cases and could be monitored by measuring the viral load in plasma.

Source Information

From the Division of Infectious Diseases (H.H.H.), the Institute for Medical Microbiology (H.H.H., T.K.), and the Divisions of Nephrology and Transplantation Immunology (M.D., J.S.) and Hematology (J.P.) and the Institute for Pathology (M.J.M.), University of Basel, Basel, Switzerland; and the Enteric, Respiratory, and Neurological Virus Laboratory, Central Public Health Laboratory, London (W.K.).

Address reprint requests to Dr. Hirsch at the Division of Infectious Diseases, Department of Internal Medicine, University Hospitals Basel, Petersgraben 4, CH-4031 Basel, Switzerland, or at hans.hirsch{at}unibas.ch.

Full Text of this Article

Related Letters:

Immunosuppression and BKV Nephropathy
Hodur D. M., Mandelbrot D., Hirsch H. H., Steiger J., Mihatsch M. J.
Extract | Full Text | PDF  
N Engl J Med 2002; 347:2079-2080, Dec 19, 2002. Correspondence

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