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Published at www.nejm.org April 10, 2003 (10.1056/NEJMoa030747)

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ABSTRACT

Background The severe acute respiratory syndrome (SARS) has recently been identified as a new clinical entity. SARS is thought to be caused by an unknown infectious agent.

Methods Clinical specimens from patients with SARS were searched for unknown viruses with the use of cell cultures and molecular techniques.

Results A novel coronavirus was identified in patients with SARS. The virus was isolated in cell culture, and a sequence 300 nucleotides in length was obtained by a polymerase-chain-reaction (PCR)-based random-amplification procedure. Genetic characterization indicated that the virus is only distantly related to known coronaviruses (identical in 50 to 60 percent of the nucleotide sequence). On the basis of the obtained sequence, conventional and real-time PCR assays for specific and sensitive detection of the novel virus were established. Virus was detected in a variety of clinical specimens from patients with SARS but not in controls. High concentrations of viral RNA of up to 100 million molecules per milliliter were found in sputum. Viral RNA was also detected at extremely low concentrations in plasma during the acute phase and in feces during the late convalescent phase. Infected patients showed seroconversion on the Vero cells in which the virus was isolated.

Conclusions The novel coronavirus might have a role in causing SARS.

Notice: Because of possible public health implications, this article was published at www.nejm.org on April 10, 2003. It will appear in the May 15 issue of the Journal.

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• DeDiego, M. L., Alvarez, E., Almazan, F., Rejas, M. T., Lamirande, E., Roberts, A., Shieh, W.-J., Zaki, S. R., Subbarao, K., Enjuanes, L. (2007). A Severe Acute Respiratory Syndrome Coronavirus That Lacks the E Gene Is Attenuated In Vitro and In Vivo. J. Virol. 81: 1701-1713 [Abstract] [Full Text]  
• Nagata, N., Iwata, N., Hasegawa, H., Fukushi, S., Yokoyama, M., Harashima, A., Sato, Y., Saijo, M., Morikawa, S., Sata, T. (2007). Participation of both Host and Virus Factors in Induction of Severe Acute Respiratory Syndrome (SARS) in F344 Rats Infected with SARS Coronavirus. J. Virol. 81: 1848-1857 [Abstract] [Full Text]  
• McCray, P. B. Jr., Pewe, L., Wohlford-Lenane, C., Hickey, M., Manzel, L., Shi, L., Netland, J., Jia, H. P., Halabi, C., Sigmund, C. D., Meyerholz, D. K., Kirby, P., Look, D. C., Perlman, S. (2007). Lethal Infection of K18-hACE2 Mice Infected with Severe Acute Respiratory Syndrome Coronavirus. J. Virol. 81: 813-821 [Abstract] [Full Text]  
• Shih, Y.-P., Chen, C.-Y., Liu, S.-J., Chen, K.-H., Lee, Y.-M., Chao, Y.-C., Chen, Y.-M. A. (2006). Identifying Epitopes Responsible for Neutralizing Antibody and DC-SIGN Binding on the Spike Glycoprotein of the Severe Acute Respiratory Syndrome Coronavirus.. J. Virol. 80: 10315-10324 [Abstract] [Full Text]  
• De Albuquerque, N., Baig, E., Ma, X., Zhang, J., He, W., Rowe, A., Habal, M., Liu, M., Shalev, I., Downey, G. P., Gorczynski, R., Butany, J., Leibowitz, J., Weiss, S. R., McGilvray, I. D., Phillips, M. J., Fish, E. N., Levy, G. A. (2006). Murine hepatitis virus strain 1 produces a clinically relevant model of severe acute respiratory syndrome in a/j mice.. J. Virol. 80: 10382-10394 [Abstract] [Full Text]  
• Louie, L., Simor, A. E., Chong, S., Luinstra, K., Petrich, A., Mahony, J., Smieja, M., Johnson, G., Gharabaghi, F., Tellier, R., Willey, B. M., Poutanen, S., Mazzulli, T., Broukhanski, G., Jamieson, F., Louie, M., Richardson, S., Ontario Laboratory Working Group for the Rapid Dia, (2006). Detection of Severe Acute Respiratory Syndrome Coronavirus in Stool Specimens by Commercially Available Real-Time Reverse Transcriptase PCR Assays. J. Clin. Microbiol. 44: 4193-4196 [Abstract] [Full Text]  
• Almazan, F., DeDiego, M. L., Galan, C., Escors, D., Alvarez, E., Ortego, J., Sola, I., Zuniga, S., Alonso, S., Moreno, J. L., Nogales, A., Capiscol, C., Enjuanes, L. (2006). Construction of a Severe Acute Respiratory Syndrome Coronavirus Infectious cDNA Clone and a Replicon To Study Coronavirus RNA Synthesis. J. Virol. 80: 10900-10906 [Abstract] [Full Text]  
• Gillim-Ross, L., Subbarao, K. (2006). Emerging Respiratory Viruses: Challenges and Vaccine Strategies. Clin. Microbiol. Rev. 19: 614-636 [Abstract] [Full Text]  
• Leung, D. T. M., van Maren, W. W. C., Chan, F. K. L., Chan, W. S., Lo, A. W. I., Ma, C. H., Tam, F. C. H., To, K. F., Chan, P. K. S., Sung, J. J. Y., Lim, P. L. (2006). Extremely Low Exposure of a Community to Severe Acute Respiratory Syndrome Coronavirus: False Seropositivity due to Use of Bacterially Derived Antigens.. J. Virol. 80: 8920-8928 [Abstract] [Full Text]  
• Kamitani, W., Narayanan, K., Huang, C., Lokugamage, K., Ikegami, T., Ito, N., Kubo, H., Makino, S. (2006). Severe acute respiratory syndrome coronavirus nsp1 protein suppresses host gene expression by promoting host mRNA degradation. Proc. Natl. Acad. Sci. USA 103: 12885-12890 [Abstract] [Full Text]  
• Zhou, M., Xu, D., Li, X., Li, H., Shan, M., Tang, J., Wang, M., Wang, F.-S., Zhu, X., Tao, H., He, W., Tien, P., Gao, G. F. (2006). Screening and Identification of Severe Acute Respiratory Syndrome-Associated Coronavirus-Specific CTL Epitopes. J. Immunol. 177: 2138-2145 [Abstract] [Full Text]  
• Lu, W., Zheng, B.-J., Xu, K., Schwarz, W., Du, L., Wong, C. K. L., Chen, J., Duan, S., Deubel, V., Sun, B. (2006). Severe acute respiratory syndrome-associated coronavirus 3a protein forms an ion channel and modulates virus release. Proc. Natl. Acad. Sci. USA 103: 12540-12545 [Abstract] [Full Text]  
• Huang, C., Ito, N., Tseng, C.-T. K., Makino, S. (2006). Severe acute respiratory syndrome coronavirus 7a accessory protein is a viral structural protein.. J. Virol. 80: 7287-7294 [Abstract] [Full Text]  
• Iacono, K. T., Kazi, L., Weiss, S. R. (2006). Both Spike and Background Genes Contribute to Murine Coronavirus Neurovirulence. J. Virol. 80: 6834-6843 [Abstract] [Full Text]  
• Kahn, J. S. (2006). Epidemiology of Human Metapneumovirus. Clin. Microbiol. Rev. 19: 546-557 [Abstract] [Full Text]  
• Spiegel, M., Schneider, K., Weber, F., Weidmann, M., Hufert, F. T. (2006). Interaction of severe acute respiratory syndrome-associated coronavirus with dendritic cells. J. Gen. Virol. 87: 1953-1960 [Abstract] [Full Text]  
• Prabakaran, P., Gan, J., Feng, Y., Zhu, Z., Choudhry, V., Xiao, X., Ji, X., Dimitrov, D. S. (2006). Structure of Severe Acute Respiratory Syndrome Coronavirus Receptor-binding Domain Complexed with Neutralizing Antibody. J. Biol. Chem. 281: 15829-15836 [Abstract] [Full Text]  
• Pasternak, A. O., Spaan, W. J. M., Snijder, E. J. (2006). Nidovirus transcription: how to make sense...?. J. Gen. Virol. 87: 1403-1421 [Abstract] [Full Text]  
• He, Y., Li, J., Heck, S., Lustigman, S., Jiang, S. (2006). Antigenic and Immunogenic Characterization of Recombinant Baculovirus-Expressed Severe Acute Respiratory Syndrome Coronavirus Spike Protein: Implication for Vaccine Design.. J. Virol. 80: 5757-5767 [Abstract] [Full Text]  
• Snijder, E. J., van der Meer, Y., Zevenhoven-Dobbe, J., Onderwater, J. J. M., van der Meulen, J., Koerten, H. K., Mommaas, A. M. (2006). Ultrastructure and Origin of Membrane Vesicles Associated with the Severe Acute Respiratory Syndrome Coronavirus Replication Complex.. J. Virol. 80: 5927-5940 [Abstract] [Full Text]  
• Chen, R.-c., Tang, X.-p., Tan, S.-y., Liang, B.-l., Wan, Z.-y., Fang, J.-q., Zhong, N. (2006). Treatment of Severe Acute Respiratory Syndrome With Glucosteroids: The Guangzhou Experience. Chest 129: 1441-1452 [Abstract] [Full Text]  
• Chu, W. C. W., Li, A. M., Ng, A. W. H., So, H.-k., Lam, W. W. M., Lo, K. L., Yeung, M.-c. A., Yau, Y.-s., Chiu, W.-k., Leung, C.-w., Ng, P.-c., Hon, K.-l., Mo, K.-w., Fok, T.-f., Ahuja, A. T. (2006). Thin-Section CT 12 Months After the Diagnosis of Severe Acute Respiratory Syndrome in Pediatric Patients.. Am. J. Roentgenol. 186: 1707-1714 [Abstract] [Full Text]  
• Chen, H., Wei, P., Huang, C., Tan, L., Liu, Y., Lai, L. (2006). Only One Protomer Is Active in the Dimer of SARS 3C-like Proteinase. J. Biol. Chem. 281: 13894-13898 [Abstract] [Full Text]  
• He, Y., Li, J., Li, W., Lustigman, S., Farzan, M., Jiang, S. (2006). Cross-Neutralization of Human and Palm Civet Severe Acute Respiratory Syndrome Coronaviruses by Antibodies Targeting the Receptor-Binding Domain of Spike Protein. J. Immunol. 176: 6085-6092 [Abstract] [Full Text]  
• Li, W., Wong, S.-K., Li, F., Kuhn, J. H., Huang, I-C., Choe, H., Farzan, M. (2006). Animal Origins of the Severe Acute Respiratory Syndrome Coronavirus: Insight from ACE2-S-Protein Interactions. J. Virol. 80: 4211-4219 [Full Text]  
• Chibo, D., Birch, C. (2006). Analysis of human coronavirus 229E spike and nucleoprotein genes demonstrates genetic drift between chronologically distinct strains.. J. Gen. Virol. 87: 1203-1208 [Abstract] [Full Text]  
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• Parker, M J, Goldman, R D (2006). Paediatric emergency department staff perceptions of infection control measures against severe acute respiratory syndrome. Emerg. Med. J. 23: 349-353 [Abstract] [Full Text]  
• Chu, L.-H. M., Choy, W.-Y., Tsai, S.-N., Rao, Z., Ngai, S.-M. (2006). Rapid peptide-based screening on the substrate specificity of severe acute respiratory syndrome (SARS) coronavirus 3C-like protease by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.. Protein Sci. 15: 699-709 [Abstract] [Full Text]  
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• Oostra, M., de Haan, C. A. M., de Groot, R. J., Rottier, P. J. M. (2006). Glycosylation of the Severe Acute Respiratory Syndrome Coronavirus Triple-Spanning Membrane Proteins 3a and M. J. Virol. 80: 2326-2336 [Abstract] [Full Text]  
• Balzarini, J., Keyaerts, E., Vijgen, L., Vandermeer, F., Stevens, M., De Clercq, E., Egberink, H., Van Ranst, M. (2006). Pyridine N-oxide derivatives are inhibitory to the human SARS and feline infectious peritonitis coronavirus in cell culture. J Antimicrob Chemother 57: 472-481 [Abstract] [Full Text]  
• Wang, L-M, Chen, Y-C, Tung, S-P, Chen, C-Y, Chang, S-C, Chiang, S-C, Lee, C-H (2006). The rationale of fever surveillance to identify patients with severe acute respiratory syndrome in Taiwan. Emerg. Med. J. 23: 202-205 [Abstract] [Full Text]  
• Peterson, L. R., Singh, K. (2006). Universal Patient Disinfection as a Tool for Infection Control: Rub-A-Dub-Dub, No Need for a Tub. Arch Intern Med 166: 274-276 [Full Text]  
• Huang, I-C., Bosch, B. J., Li, F., Li, W., Lee, K. H., Ghiran, S., Vasilieva, N., Dermody, T. S., Harrison, S. C., Dormitzer, P. R., Farzan, M., Rottier, P. J. M., Choe, H. (2006). SARS Coronavirus, but Not Human Coronavirus NL63, Utilizes Cathepsin L to Infect ACE2-expressing Cells. J. Biol. Chem. 281: 3198-3203 [Abstract] [Full Text]  
• Chen, Y.-M. A., Liang, S.-Y., Shih, Y.-P., Chen, C.-Y., Lee, Y.-M., Chang, L., Jung, S.-Y., Ho, M.-S., Liang, K.-Y., Chen, H.-Y., Chan, Y.-J., Chu, D.-C. (2006). Epidemiological and Genetic Correlates of Severe Acute Respiratory Syndrome Coronavirus Infection in the Hospital with the Highest Nosocomial Infection Rate in Taiwan in 2003. J. Clin. Microbiol. 44: 359-365 [Abstract] [Full Text]  
• Zhong, X., Guo, Z., Yang, H., Peng, L., Xie, Y., Wong, T.-Y., Lai, S.-T., Guo, Z. (2006). Amino terminus of the SARS coronavirus protein 3a elicits strong, potentially protective humoral responses in infected patients. J. Gen. Virol. 87: 369-373 [Abstract] [Full Text]  
• Broer, R., Boson, B., Spaan, W., Cosset, F.-L., Corver, J. (2006). Important Role for the Transmembrane Domain of Severe Acute Respiratory Syndrome Coronavirus Spike Protein during Entry. J. Virol. 80: 1302-1310 [Abstract] [Full Text]  
• Xu, J., Qi, L., Chi, X., Yang, J., Wei, X., Gong, E., Peh, S., Gu, J. (2006). Orchitis: A Complication of Severe Acute Respiratory Syndrome (SARS). Biol. Reprod. 74: 410-416 [Abstract] [Full Text]  
• Yu, C-M, Wong, R S-M, Wu, E B, Kong, S-L, Wong, J, Yip, G W-K, Soo, Y O Y, Chiu, M L S, Chan, Y-S, Hui, D, Lee, N, Wu, A, Leung, C-B, Sung, J J-Y (2006). Cardiovascular complications of severe acute respiratory syndrome. Postgrad. Med. J. 82: 140-144 [Abstract] [Full Text]  
• Huang, C., Narayanan, K., Ito, N., Peters, C. J., Makino, S. (2006). Severe Acute Respiratory Syndrome Coronavirus 3a Protein Is Released in Membranous Structures from 3a Protein-Expressing Cells and Infected Cells. J. Virol. 80: 210-217 [Abstract] [Full Text]  
• Ho, H.-T., Chang, M.-S., Wei, T.-Y., Hsieh, W.-S., Hung, C.-C., Yang, H.-M., Lu, Y.-T. (2006). Colonization of Severe Acute Respiratory Syndrome-Associated Coronavirus Among Health-Care Workers Screened by Nasopharyngeal Swab. Chest 129: 95-101 [Abstract] [Full Text]  
• Galan, C., Enjuanes, L., Almazan, F. (2005). A Point Mutation within the Replicase Gene Differentially Affects Coronavirus Genome versus Minigenome Replication. J. Virol. 79: 15016-15026 [Abstract] [Full Text]  
• Sims, A. C., Baric, R. S., Yount, B., Burkett, S. E., Collins, P. L., Pickles, R. J. (2005). Severe Acute Respiratory Syndrome Coronavirus Infection of Human Ciliated Airway Epithelia: Role of Ciliated Cells in Viral Spread in the Conducting Airways of the Lungs. J. Virol. 79: 15511-15524 [Abstract] [Full Text]  
• Weiss, S. R., Navas-Martin, S. (2005). Coronavirus Pathogenesis and the Emerging Pathogen Severe Acute Respiratory Syndrome Coronavirus. Microbiol. Mol. Biol. Rev. 69: 635-664 [Abstract] [Full Text]  
• Yount, B., Roberts, R. S., Sims, A. C., Deming, D., Frieman, M. B., Sparks, J., Denison, M. R., Davis, N., Baric, R. S. (2005). Severe Acute Respiratory Syndrome Coronavirus Group-Specific Open Reading Frames Encode Nonessential Functions for Replication in Cell Cultures and Mice. J. Virol. 79: 14909-14922 [Abstract] [Full Text]  
• Hsieh, P.-K., Chang, S. C., Huang, C.-C., Lee, T.-T., Hsiao, C.-W., Kou, Y.-H., Chen, I-Y., Chang, C.-K., Huang, T.-H., Chang, M.-F. (2005). Assembly of Severe Acute Respiratory Syndrome Coronavirus RNA Packaging Signal into Virus-Like Particles Is Nucleocapsid Dependent. J. Virol. 79: 13848-13855 [Abstract] [Full Text]  
• Vijgen, L., Keyaerts, E., Moes, E., Maes, P., Duson, G., Van Ranst, M. (2005). Development of One-Step, Real-Time, Quantitative Reverse Transcriptase PCR Assays for Absolute Quantitation of Human Coronaviruses OC43 and 229E. J. Clin. Microbiol. 43: 5452-5456 [Abstract] [Full Text]  
• Chan, K. H., Cheng, V. C. C., Woo, P. C. Y., Lau, S. K. P., Poon, L. L. M., Guan, Y., Seto, W. H., Yuen, K. Y., Peiris, J. S. M. (2005). Serological Responses in Patients with Severe Acute Respiratory Syndrome Coronavirus Infection and Cross-Reactivity with Human Coronaviruses 229E, OC43, and NL63. CVI 12: 1317-1321 [Abstract] [Full Text]  
• Mahony, J. B., Richardson, S. (2005). Molecular Diagnosis of Severe Acute Respiratory Syndrome: The State of the Art. J. Mol. Diagn. 7: 551-559 [Abstract] [Full Text]  
• Li, A M, Ng, P C (2005). Severe acute respiratory syndrome (SARS) in neonates and children. Arch. Dis. Child. Fetal Neonatal Ed. 90: F461-F465 [Abstract] [Full Text]  
• Grace, S. L., Hershenfield, K., Robertson, E., Stewart, D. E. (2005). The Occupational and Psychosocial Impact of SARS on Academic Physicians in Three Affected Hospitals. Psychosomatics 46: 385-391 [Abstract] [Full Text]  
• Yip, S. P., To, S. S. T., Leung, P. H.M., Cheung, T. S., Cheng, P. K.C., Lim, W. W.L. (2005). Use of Dual TaqMan Probes to Increase the Sensitivity of 1-Step Quantitative Reverse Transcription-PCR: Application to the Detection of SARS Coronavirus. Clin. Chem. 51: 1885-1888 [Full Text]