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Molecular Medicine
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Volume 332:589-591 March 2, 1995 Number 9
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Fine Structure of a Gene — DNA Sequencing

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A molecular voyager entering the nucleus of a human cell in search of a gene would be hard pressed to discern its location, buried in the tangle of chromosomal DNA. Only a painstaking examination of the nucleotide sequence along the helical DNA strands would reveal those rare stretches of nucleotides that could be read as a code for the structure of a protein. We do not yet possess the means to delve directly into the nucleus and search for genes in their natural setting, but by molecular cloning we can isolate and amplify relatively short stretches of chromosomal DNA and . . . [Full Text of this Article]

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