FREE NEJM E-TOC    HOME   |   SUBSCRIBE   |   CURRENT ISSUE   |   PAST ISSUES   |   COLLECTIONS   |   Search Term  Advanced Search

Sign in | Get NEJM's E-Mail Table of Contents — Free | Subscribe

Previous Volume 333:525 August 24, 1995 Number 8 Next

	Since this article has no abstract, we have provided an extract of the first 100 words of the full text and any section headings.

	Full Text Purchase this article Add to Personal Archive Add to Citation Manager Notify a Friend E-mail When Cited PubMed Citation

To the Editor: By injecting sperm into the cytoplasm of an oocyte¹ it is now possible to achieve fertilization and pregnancy in cases of obstructive azoospermia or severe oligozoospermia and asthenospermia after obtaining sperm directly from the epididymis or testis.² Still, many patients with nonobstructive azoospermia cannot benefit from these techniques. The recently reported birth of normal mice after the electrofusion of oocytes with round spermatids, the youngest male germ cells to have a set of haploid chromosomes,³ has suggested a new approach to the treatment of this condition.

In our sperm-injection program, we have seen 12 men with unexplained . . . [Full Text of this Article]

References

This article has been cited by other articles:

• Lee, D. R., Kim, K.-S., Yang, Y. H., Oh, H. S., Lee, S. H., Chung, T. G., Cho, J. H., Kim, H. J., Yoon, T. K., Cha, K. Y. (2006). Isolation of male germ stem cell-like cells from testicular tissue of non-obstructive azoospermic patients and differentiation into haploid male germ cells in vitro. Hum Reprod 21: 471-476 [Abstract] [Full Text]  
• Tsujimura, A., Matsumiya, K., Miyagawa, Y., Tohda, A., Miura, H., Nishimura, K., Koga, M., Takeyama, M., Fujioka, H., Okuyama, A. (2002). Conventional multiple or microdissection testicular sperm extraction: a comparative study. Hum Reprod 17: 2924-2929 [Abstract] [Full Text]  
• De Rycke, M., Liebaers, I., Van Steirteghem, A. (2002). Epigenetic risks related to assisted reproductive technologies: Risk analysis and epigenetic inheritance. Hum Reprod 17: 2487-2494 [Abstract] [Full Text]  
• Coskun, S., Tbakhi, A., Jaroudi, K. A., Uzumcu, M., Merdad, T. A., Al-Hussein, K. A. (2002). Flow cytometric ploidy analysis of testicular biopsies from sperm-negative wet preparations. Hum Reprod 17: 977-983 [Abstract] [Full Text]  
• Sousa, M., Cremades, N., Alves, C., Silva, J., Barros, A. (2002). Developmental potential of human spermatogenic cells co-cultured with Sertoli cells. Hum Reprod 17: 161-172 [Abstract] [Full Text]  
• Kang-Decker, N., Mantchev, G. T., Juneja, S. C., McNiven, M. A., van Deursen, J. M. A. (2001). Lack of Acrosome Formation in Hrb-Deficient Mice. Science 294: 1531-1533 [Abstract] [Full Text]  
• Cremades, N., Sousa, M., Bernabeu, R., Barros, A. (2001). Developmental potential of elongating and elongated spermatids obtained after in-vitro maturation of isolated round spermatids. Hum Reprod 16: 1938-1944 [Abstract] [Full Text]  
• Johnson, L., Staub, C., Neaves, W. B., Yanagimachi, R. (2001). Live human germ cells in the context of their spermatogenic stages. Hum Reprod 16: 1575-1582 [Abstract] [Full Text]  
• Ogonuki, N., Sankai, T., Yagami, K., Shikano, T., Oda, S., Miyazaki, S., Ogura, A. (2001). Activity of a Sperm-Borne Oocyte-Activating Factor in Spermatozoa and Spermatogenic Cells from Cynomolgus Monkeys and Its Localization after Oocyte Activation. Biol. Reprod. 65: 351-357 [Abstract] [Full Text]  
• Amer, M., Elnasser, T. A., Haggar, S. E., Mostafa, T., Abdel-Malak, G., Zohdy, W. (2001). May-Grunwald-Giemsa stain for detection of spermatogenic cells in the ejaculate: a simple predictive parameter for successful testicular sperm retrieval. Hum Reprod 16: 1427-1432 [Abstract] [Full Text]  
• Ziyyat, A., Lefevre, A. (2001). Differential gene expression in pre-implantation embryos from mouse oocytes injected with round spermatids or spermatozoa. Hum Reprod 16: 1449-1456 [Abstract] [Full Text]  
• Foresta, C., Moro, E., Ferlin, A. (2001). Y Chromosome Microdeletions and Alterations of Spermatogenesis. Endocr. Rev. 22: 226-239 [Abstract] [Full Text]  
• Steger, K., Failing, K., Klonisch, T., Behre, H. M., Manning, M., Weidner, W., Hertle, L., Bergmann, M., Kliesch, S. (2001). Round spermatids from infertile men exhibit decreased protamine-1 and -2 mRNA. Hum Reprod 16: 709-716 [Abstract] [Full Text]  
• Grundy, R, Gosden, R G, Hewitt, M, Larcher, V, Leiper, A, Spoudeas, H A, Walker, D, Wallace, W H B (2001). Personal practice: Fertility preservation for children treated for cancer (1): scientific advances and research dilemmas. Arch. Dis. Child. 84: 355-359 [Full Text]  
• Yazawa, H., Yanagida, K., Katayose, H., Hayashi, S., Sato, A. (2000). Comparison of oocyte activation and Ca2+ oscillation-inducing abilities of round/elongated spermatids of mouse, hamster, rat, rabbit and human assessed by mouse oocyte activation assay. Hum Reprod 15: 2582-2590 [Abstract] [Full Text]  
• Tesarik, J., Balaban, B., Isiklar, A., Alatas, C., Urman, B., Aksoy, S., Mendoza, C., Greco, E. (2000). In-vitro spermatogenesis resumption in men with maturation arrest: relationship with in-vivo blocking stage and serum FSH. Hum Reprod 15: 1350-1354 [Abstract] [Full Text]  
• Tsai, M. C., Takeuchi, T., Bedford, J.M., Reis, M. M., Rosenwaks, Z., Palermo, G. D. (2000). Alternative sources of gametes: reality or science fiction?. Hum Reprod 15: 988-998 [Abstract] [Full Text]  
• Kay, V.J., Irvine, D.S. (2000). Successful in-vitro fertilization pregnancy with spermatozoa from a patient with Kartagener's syndrome: Case Report. Hum Reprod 15: 135-138 [Abstract] [Full Text]  
• Johnson, L., Neaves, W. B., Barnard, J. J., Keillor, G. E., Brown, S. W., Yanagimachi, R. (1999). A Comparative Morphological Study of Human Germ Cells In Vitro or In Situ Within Seminiferous Tubules. Biol. Reprod. 61: 927-934 [Abstract] [Full Text]  
• Moomjy, M., Colombero, L. T., Veeck, L. L., Rosenwaks, Z., Palermo, G. D. (1999). Sperm integrity is critical for normal mitotic division and early embryonic development. Mol Hum Reprod 5: 836-844 [Abstract] [Full Text]  
• Prapas, Y., Chatziparasidou, A., Vanderzwalmen, P., Nijs, M., Prapas, N., Lejeune, B., Vlassis, G., Schoysman, R. (1999). Spermatid injection: Reconsidering spermatid injection. Hum Reprod 14: 2186-2188 [Full Text]  
• Sutovsky, P., Ramalho-Santos, J., Moreno, R. D., Oko, R., Hewitson, L., Schatten, G. (1999). On-stage selection of single round spermatids using a vital, mitochondrion-specific fluorescent probe MitoTrackerTM and high resolution differential interference contrast microscopy. Hum Reprod 14: 2301-2312 [Abstract] [Full Text]  
• Sofikitis, N., Ono, K., Yamamoto, Y., Papadopoulos, H., Miyagawa, I. (1999). Influence of the male reproductive tract on the reproductive potential of round spermatids abnormally released from the seminiferous epithelium. Hum Reprod 14: 1998-2006 [Abstract] [Full Text]  
• Yamamoto, Y., Sofikitis, N., Miyagawa, I. (1999). Ooplasmic injections of rabbit round spermatid nuclei or intact round spermatids from fresh, cryopreserved and cryostored samples. Hum Reprod 14: 1506-1515 [Abstract] [Full Text]  
• Aslam, I., Fishel, S. (1999). Evaluation of the fertilization potential of freshly isolated, in-vitro cultured and cryopreserved human spermatids by injection into hamster oocytes. Hum Reprod 14: 1528-1533 [Abstract] [Full Text]  
• Pang, M.G., Hoegerman, S.F., Cuticchia, A.J., Moon, S.Y., Doncel, G.F., Acosta, A.A., Kearns, W.G. (1999). Detection of aneuploidy for chromosomes 4, 6, 7, 8, 9, 10, 11, 12, 13, 17, 18, 21, X and Y by fluorescence in-situ hybridization in spermatozoa from nine patients with oligoasthenoteratozoospermia undergoing intracytoplasmic sperm injection. Hum Reprod 14: 1266-1273 [Abstract] [Full Text]  
• Sousa, M., Barros, A., Takahashi, K., Oliveira, C., Silva, J., Tesarik, J. (1999). Clinical efficacy of spermatid conception: analysis using a new spermatid classification scheme. Hum Reprod 14: 1279-1286 [Abstract] [Full Text]  
• Cremades, N., Bernabeu, R., Barros, A., Sousa, M. (1999). In-vitro maturation of round spermatids using co-culture on Vero cells. Hum Reprod 14: 1287-1293 [Abstract] [Full Text]  
• Jurisicova, A., Lopes, S., Meriano, J., Oppedisano, L., Casper, R. F., Varmuza, S. (1999). DNA damage in round spermatids of mice with a targeted disruption of the Pp1c{gamma} gene and in testicular biopsies of patients with non-obstructive azoospermia. Mol Hum Reprod 5: 323-330 [Abstract] [Full Text]  
• Gandini, L., Lenzi, A., Lombardo, F., Pacifici, R., Dondero, F. (1999). Immature germ cell separation using a modified discontinuous Percoll gradient technique in human semen. Hum Reprod 14: 1022-1027 [Abstract] [Full Text]  
• Sakurai, A., Oda, S., Kuwabara, Y., Miyazaki, S. (1999). Fertilization, embryonic development, and offspring from mouse eggs injected with round spermatids combined with Ca2+ oscillation-inducing sperm factor. Mol Hum Reprod 5: 132-138 [Abstract] [Full Text]  
• Hammadeh, M.E., Al-Hasani, S., Doerr, S., Stieber, M., Rosenbaum, P., Schmidt, W., Diedrich, K. (1999). Comparison between chromatin condensation and morphology from testis biopsy extracted and ejaculated spermatozoa and their relationship to ICSI outcome. Hum Reprod 14: 363-367 [Abstract] [Full Text]  
• Ziyyat, A., Lassalle, B., Testart, J., Briot, P., Amar, E., Finaz, C., Lefevre, A. (1999). Flow cytometry isolation and reverse transcriptase–polymerase chain reaction characterization of human round spermatids in infertile patients. Hum Reprod 14: 379-387 [Abstract] [Full Text]  
• Lassalle, B., Ziyyat, A., Testart, J., Finaz, C., Lefevre, A. (1999). Flow cytometric method to isolate round spermatids from mouse testis. Hum Reprod 14: 388-394 [Abstract] [Full Text]  
• Bahadur, G., Ralph, D. (1999). Gonadal tissue cryopreservation in boys with paediatric cancers. Hum Reprod 14: 11-17 [Abstract] [Full Text]  
• Ogura, A., Suzuki, O., Tanemura, K., Mochida, K., Kobayashi, Y., Matsuda, J. (1998). Development of normal mice from metaphase I oocytes fertilized with primary spermatocytes. Proc. Natl. Acad. Sci. USA 95: 5611-5615 [Abstract] [Full Text]  
• Schlegel, P. N., Girardi, S. K. (1997). In Vitro Fertilization for Male Factor Infertility. J. Clin. Endocrinol. Metab. 82: 709-716 [Full Text]