To the Editor: Foreman et al. (Jan. 16 issue)1 report the propagationof Kaposi's sarcomaassociated herpesvirus (KSHV) fromKaposi's sarcomaderived cell lines, using cells of thehuman embryonal-kidney epithelial-cell line as targets for infection.In three experiments, we attempted to propagate KSHV directlyfrom Kaposi's sarcoma tumors by culturing fresh, finely mincedtissue with 293 cells, some of which were kindly given us bythe University of Michigan group and some of which were purchasedfrom the American Tissue Culture Collection (ATCC), Rockville,Maryland.
Within two to three days of coculture, the 293 cells from theMichigan group showed marked . . . [Full Text of this Article]
Ablashi, D. V., Chatlynne, L. G., Whitman, J. E. Jr., Cesarman, E.
(2002). Spectrum of Kaposi's Sarcoma-Associated Herpesvirus, or Human Herpesvirus 8, Diseases. Clin. Microbiol. Rev.
15: 439-464
[Abstract][Full Text]
Mori, Y., Dhepakson, P., Shimamoto, T., Ueda, K., Gomi, Y., Tani, H., Matsuura, Y., Yamanishi, K.
(2000). Expression of Human Herpesvirus 6B rep within Infected Cells and Binding of Its Gene Product to the TATA-Binding Protein In Vitro and In Vivo. J. Virol.
74: 6096-6104
[Abstract][Full Text]
Orenstein, J. M., Herndier, B., Aster, J. C., Jones, D.
(1999). Appearance of Human Herpesvirus 8 on Electron Microscopy. NEJM
340: 62-64
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