Prevalence of the Rickettsial Agent of Human Granulocytic Ehrlichiosis in Ticks from a Hyperendemic Focus of Lyme Disease

doi:10.1056/NEJM199707033370111

Volume 337:49-50		July 3, 1997		Number 1

Prevalence of the Rickettsial Agent of Human Granulocytic Ehrlichiosis in Ticks from a Hyperendemic Focus of Lyme Disease

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To the Editor: Human granulocytic ehrlichiosis, a newly discoveredtick-borne infection originally described in the upper Midwest,¹has recently been reported in 29 patients from Westchester County,New York.² We used the polymerase chain reaction (PCR) to examine173 host-seeking ticks (Ixodes scapularis) collected from vegetationin Westchester County for the presence of the agent of humangranulocytic ehrlichiosis, as well as the agent of Lyme disease,Borrelia burgdorferi. After extracting DNA from dissected tissues,we used the 23S ribosomal DNA (rDNA) primers of Schwartz etal.³ for B. burgdorferi and the 16S rDNA primers of Pancholi. . . [Full Text of this Article]

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