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Correspondence
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Volume 345:1067-1068 October 4, 2001 Number 14
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Selection of Spermatozoa with Normal Nuclei to Improve the Pregnancy Rate with Intracytoplasmic Sperm Injection

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To the Editor: Intracytoplasmic injection of sperm is the recommended treatment for male infertility, associated with an average pregnancy rate per cycle of about 30 percent.1 Although sperm count and motility were found to have no effect on the outcome of intracytoplasmic sperm injection,2 scanning and transmission electron microscopy indicated that the achievement of pregnancy may depend on normal morphology of the sperm nucleus.3

We therefore introduced the motile-sperm organelle-morphology examination (MSOME), by which the fine nuclear morphology of motile spermatozoa is examined in real time with an inverted light microscope equipped with high-power differential interference contrast (Nomarski/DIC) optics (magnification, . . . [Full Text of this Article]

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