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Previous Volume 349:1149-1156 September 18, 2003 Number 12 Next

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The ability to discern the molecular "fingerprint" (genotype) of Mycobacterium tuberculosis isolates has revolutionized our understanding of the transmission of tuberculosis. In this review, we summarize the main methods of determining the genotype and discuss the relevance of genotyping to the control and understanding of the pathogenesis of tuberculosis.

Genotyping Methods

The standard approach to genotyping M. tuberculosis isolates is restriction-fragment–length polymorphism (RFLP) analysis of the distribution of the insertion sequence IS6110 in different strains,¹ and large data bases of IS6110-based genotypes are available (Figure 1). Isolates from patients infected with epidemiologically unrelated strains of tuberculosis have different RFLP patterns, . . . [Full Text of this Article]

Genotyping for Clinical Management

Insights into the Transmission of Tuberculosis

Use of Genotyping by Tuberculosis-Control Programs

Identification of Groups at Increased Risk for Tuberculosis

Improving Investigations of Contacts

Evaluation of Tuberculosis Programs

Future Uses of Genotyping

Relevance of Genotyping to the Pathogenesis of Tuberculosis

Microbial Factors That Favor Transmission

Drug Resistance and Disease Transmission

The Frequency of Reinfection

Source Information

From the Center for Pulmonary and Infectious Disease Control (CPIDC), Departments of Medicine and Microbiology and Immunology, University of Texas Health Center, Tyler (P.F.B.); and the Departments of Anatomy and Neurobiology, University of Arkansas for Medical Sciences and Medical Research Service, Central Arkansas Veterans Healthcare System, Little Rock (M.D.C.).

Address reprint requests to Dr. Barnes at the CPIDC, University of Texas Health Center, 11937 U.S. Hwy. 271, Tyler, TX 75708-3154, or at peter.barnes@uthct.edu.

Related Letters:

Molecular Epidemiology of Tuberculosis
Rosenblum L. S., Navin T. R., Crawford J. T., Portero J. L., Rubio M.
Extract | Full Text | PDF  
N Engl J Med 2003; 349:2364, Dec 11, 2003. Correspondence

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