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Previous Volume 350:1740-1745 April 22, 2004 Number 17 Next

	Since this article has no abstract, we have provided an extract of the first 100 words of the full text and any section headings.

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The outbreak of severe acute respiratory syndrome (SARS) in Singapore ended in late May 2003.¹ The Centers for Disease Control and Prevention (CDC) removed its travel alerts for Toronto, Hong Kong, China, and Taiwan shortly thereafter.² We report the first case of SARS to occur in Singapore after the initial worldwide outbreak ended. Our report documents the transmission of SARS in a laboratory setting.

Case Report

A 27-year-old graduate student in microbiology at a local university was admitted to Singapore General Hospital on September 3, 2003, with fever. In July and August 2003, he had worked with a nonattenuated strain of West . . . [Full Text of this Article]

Methods

Epidemiologic Investigations

Laboratory Investigations

Nucleic Acid Extraction

RT-PCR

Serologic Analysis

Viral Culture

Sequence Analysis of the SARS-CoV Viral Genome

Results

Epidemiologic Investigations

PCR, Serologic Analyses, and Viral Culture

Genome Sequence Analysis of SARS-CoV from the Patient

Discussion

Source Information

From the Department of Infectious Diseases, Tan Tock Seng Hospital (P.L.L., B.A., Y.-S.L.); the Departments of Medicine (A.K.) and Pathology (K.P.C., S.Y.S-T., L.O., X.B.), Singapore General Hospital; the Ministry of Health (G.G., L.J., P.L.O., C.S.K.); the Genome Institute of Singapore (C.W.W., L.W.S., Y.R., L.D.M., V.B.V.); and DSO National Laboratories (L.C.N.) — all in Singapore; and the International Emerging Infections Program, Centers for Disease Control and Prevention, Nonthaburi, Thailand (S.J.O.).

Address reprint requests to Dr. Lim at the Department of Infectious Diseases, Tan Tock Seng Hospital, 11 Jalan Tan Tock Seng, Singapore 308433, or at pllim@post.harvard.edu.

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