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NEJM -- Selzer RR et al. Adverse Effect of Nitrous Oxide in a Child with 5,10-Methylenetetrahydrofolate Reductase Deficiency. 349(1):45-50. Supplementary Appendixes 1-3 Article: Selzer RR et al. Adverse Effect of Nitrous Oxide in a Child with 5,10-Methylenetetrahydrofolate Reductase Deficiency. N Engl J Med 2003;349(1):45-50.


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 Supplementary Appendix 1. Oligonucleotide Primers Used for Amplification and Sequencing of MTHFR Exons from Genomic DNA.*



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 Supplementary Appendix 2. Primers Used to Sequence the Complementary DNA Transcript.




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 Supplementary Appendix 3. Primers Used to Amplify Complementary DNA as Seven Overlapping Fragments.
 

 

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