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Supplementary Appendix 2. Western Blot Analysis of Materials Eluted from Anti–Mannan-Binding Lectin–Coated Wells after Incubation with Plasma from the Patient.
The blots were developed with antibody against MAp19 and mannan-binding lectin–associated serine protease 2 (MASP-2) (Panel A) and antibody against MASP-1 (Panel B). In each panel, lane 1 shows plasma from the patient, lane 2 plasma from a subject with normal levels of mannan-binding lectin (MBL), and lane 3 plasma from a subject with MBL deficiency.
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