Pagès et al. 353 (25): 2654, Figure 3 December 22, 2005

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Figure 3. Large-Scale Four-Color Flow-Cytometric Analysis of the Cell Populations and Immune-Cell Subpopulations in Freshly Resected Tumors from 39 Patients with Colorectal Carcinoma.

Early metastatic invasion was defined by the presence of vascular emboli, lymphatic invasion, and perineural invasion (collectively referred to as VELIPI), alone or in combination. Panel A shows the 410 combinations of surface markers measured by means of a fluorescence-activated cell sorter and subsequently plotted from the minimal level of expression (blue) to the maximal (red); gray areas represent analyses that were not done. Panel B shows the hierarchical clustering of the 65 combinations of markers that differed significantly between VELIPI-negative and VELIPI-positive tumors (P<0.05). Panel C shows the T-cell differentiation process with the use of the markers CD45RO, CCR7, CD28, and CD27. Cells were analyzed in VELIPI-postive tumors and VELIPI-negative tumors and expressed as the mean (+SE) percentage of the total cells present within the tumor. Panel D shows the CD8+ T-cell subpopulations from naive to effector T cells with the use of the markers CD3, CD8, CCR7, and CD45RO, represented as the mean (+SE) percentage of the total cells within the tumor and as the factor increase among VELIPI-negative tumors, as compared with VELIPI-positive tumors. Immune-cell subpopulations (percentage of positive cells in the total population isolated from the tumor and with the CD3+ T-cell population) analyzed as a percentage of total cells reflect their density within the tumor. The Mann–Whitney test was used for statistical analyses.

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