Pagès et al. 353 (25): 2654, Figure 4 December 22, 2005

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Figure 4. Tissue Microarray (Panel A); Correlation between Optical and Automatic Counts of CD45RO+ Cells (Panel B); Mean (+SE) Density of CD45RO+ Cells According to the Presence or Absence of Early Metastatic Invasion, Nodal (N) Stage, and Metastatis (M) Stage (Panel C); and Overall and Disease-free Survival According to the Presence of a High or Low Density of CD45RO+ Cells within the Tumor (Panel D).

Early metastatic invasion was defined by the presence of vascular emboli, lymphatic invasion, and perineural invasion (collectively referred to as VELIPI), alone or in combination. Panel A shows the tissue microarrays. Four cores were obtained (two from the center and two from the invasive margin of the tumor) from the tumor specimens from 415 patients with colorectal cancer. Panel A shows an enlargement of a spot and an enlargement of CD45RO+ cells, as well as the digital image captured by the camera and analyzed by the image software (Spot Browser), with tissue represented in yellow and CD45RO+ cells represented in red. Panel B shows the correlation between optical and automatic counts of CD45RO+ cells in 100 randomly selected patients. Panel C shows the mean (±SE) numbers of CD45RO+ cells in the various groups of patients according to the VELIPI, N, and M status. N and M stages were determined according to the TNM staging system of the Union Internationale contre le Cancer.¹⁶ NA denotes not applicable. The Mann–Whitney test was used for statistical analyses. Panel D shows Kaplan–Meier curves for overall survival and disease-free survival among 160 patients with tumors that had a high density of CD45RO+ cells (at least 250 per square millimeter) and 176 patients with tumors that had a low density of CD45RO+ cells (fewer than 250 per square millimeter).

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