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Previous Volume 360:2583-2584 June 11, 2009 Number 24 Next

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To the Editor: Human infection with the H5N1 or H9N2 avian influenza virus has been reported in the city of Guangzhou in southern China.^1,2 To assess the risk of avian influenza virus infection among humans, we conducted a serologic surveillance study in Guangzhou. A total of 2191 healthy persons were recruited from 230 workplaces and seven types of occupations from March 2007 through July 2008. This study was approved by the ethics committee of the Guangzhou Center for Disease Control and Prevention, and written informed consent was obtained from the subjects.

Serum samples obtained from these subjects were assayed for antibodies against H5 and H9 avian influenza virus with a hemagglutination-inhibition assay containing 4 hemagglutination units of inactivated H5N2 virus strain A/Turkey/England/N28/1973 and H9N2 virus strain A/Chicken/Shandong/6/1996. The results were further confirmed, as described previously,^2,3 by means of a neutralization assay against 100 tissue-culture infective doses of H5N1 strains A/Hong Kong/486/1997 and A/Vietnam/1194/2004 and H9N2 strain HK/2108/2003.

The prevalence of anti-H5 antibodies was 0.2% and the prevalence of anti-H9 antibodies was 4.5% (Table 1). All anti-H5–positive persons were poultry retailers or wholesalers, but anti-H9–positive persons were identified in all study groups in this cohort. It has been reported that H5N1 and H9N2 viruses were cocirculating among poultry and humans in markets.⁴ Our results show that the positive rate of anti-H5 antibodies was much lower than that of anti-H9 antibodies among poultry retailers (0.8% vs. 15.5%, P<0.001) and among wholesalers (0.8% vs. 6.6%, P=0.001). This difference may be attributed to the wide use of H5 vaccine in poultry, whereas vaccination against H9 is not available. In addition, H9 avian influenza virus infection in poultry is usually asymptomatic and associated with virus shedding. Given that reassortant of H9N2 with other subtypes of avian influenza virus has been reported to occur in southern China,⁵ our finding highlights the potential risk of H9 avian influenza virus to public health.

View this table: [in this window] [in a new window]   Table 1. Results of a Serologic Surveillance Study of Human Infection with H5 or H9 Avian Influenza Virus.

 
The anti-H9–positive rate was highest among poultry retailers (15.5%); this rate was significantly higher than the rate among the other six groups (P0.006) (Table 1). The significantly higher anti-H9–positive rate among poultry retailers than among other retailers in the same markets (15.5% vs. 1.8%, P<0.001) may be due to the direct contact of poultry retailers with live birds, particularly during the slaughter of poultry. It also suggests that transmission of H9 avian influenza virus in the markets was mainly through poultry-to-human but not human-to-human contact. The anti-H9–positive rate was also significantly higher among poultry retailers than among wholesalers (15.5% vs. 6.6%, P=0.002). This difference may be attributed to the fact that retailers purchased different species of poultry from multiple wholesalers; thus, control of poultry-to-poultry transmission of avian influenza virus in markets with live poultry may be important to the prevention of avian influenza virus infection in humans.

Ming Wang, M.D.
Chuan-Xi Fu, Ph.D.
Guangzhou Center for Disease Control and Prevention
Guangzhou 510080, China
wangming{at}gzcdc.org.cn

Bo-Jian Zheng, M.D., Ph.D.
University of Hong Kong
Hong Kong, China

References

1. Wang M, Di B, Zhou DH, et al. Food markets with live birds as source of avian influenza. Emerg Infect Dis 2006;12:1773-1775. [Web of Science][Medline]
2. Guo YJ, Li J, Cheng X, Wang M, et al. Discovery of man infected by avian influenza virus. Chin J Exp Clin Virol 1999;13:105-8.
3. Zheng BJ, Chan KW, Lin YP, et al. Delayed antiviral plus immunomodulator treatment still reduces mortality in mice infected by high inoculum of influenza A/H5N1 virus. Proc Natl Acad Sci U S A 2008;105:8091-8096. [Free Full Text]
4. Shortridge KF, Zhou NN, Guan Y, et al. Characterization of avian H5N1 influenza viruses from poultry in Hong Kong. Virology 1998;252:331-342. [CrossRef][Web of Science][Medline]
5. Xu KM, Li KS, Smith GJ, et al. Evolution and molecular epidemiology of H9N2 influenza A viruses from quail in southern China, 2000 to 2005. J Virol 2007;81:2635-2645. [Free Full Text]

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