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Previous Volume 331:1459 November 24, 1994 Number 21 Next

	Since this article has no abstract, we have provided an extract of the first 100 words of the full text and any section headings.

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To the Editor: In a transplant recipient with disseminated amebic infection (July 14 issue),¹ Slater et al. reported that organisms were initially overlooked on examination of the skin-biopsy specimen stained with hematoxylin and eosin. When we suspect acanthamoeba infection, we use Calcofluor white M2R, an inexpensive (about 10 cents per test), colorless compound that is widely available commercially (from American Cyanamid, Difco, Polysciences, Remel, and Sigma). By binding to polysaccharides such as cellulose and chitin, this fluorochrome highlights amebic cysts, microsporidial spores, filamentous fungi, yeasts, Pneumocystis carinii, algae, and plant-containing foreign material.

Calcofluor white (or a related toluylene derivative) is . . . [Full Text of this Article]

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