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Previous Volume 332:1521-1522 June 1, 1995 Number 22 Next

	Since this article has no abstract, we have provided an extract of the first 100 words of the full text and any section headings.

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To the Editor: Immunohistochemical techniques are commonly used to detect antigens of therapeutic or prognostic importance in patients with cancer. Some technical problems in their use are well recognized, such as antigen masking by fixatives.¹ We recently encountered another potential problem — antigen loss in slides stored at room temperature.

While studying p53 gene expression in breast cancers using immunohistochemical analysis of formalin-fixed, paraffin-embedded sections, we observed diminished reactivity over time on our p53-positive control slides. These slides had been cut from a paraffin block containing a p53-positive breast cancer and stored at room temperature for up to two months.

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References

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More on p53 Antigen Loss in Stored Paraffin Slides
Kato J., Sakamaki S., Niitsu Y., Prioleau J. E., Schnitt S. J.
Extract | Full Text  
N Engl J Med 1995; 333:1507-1508, Nov 30, 1995. Correspondence

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