FREE NEJM E-TOC    HOME   |   SUBSCRIBE   |   CURRENT ISSUE   |   PAST ISSUES   |   COLLECTIONS   |   Search Term  Advanced Search

Sign in | Get NEJM's E-Mail Table of Contents — Free | Subscribe

Previous Volume 333:487-493 August 24, 1995 Number 8 Next

	Full Text PDF Add to Personal Archive Add to Citation Manager Notify a Friend E-mail When Cited PubMed Citation

ABSTRACT

Background In severe congenital neutropenia the maturation of myeloid progenitor cells is arrested. The myelodysplastic syndrome and acute myeloid leukemia develop in some patients with severe congenital neutropenia. Abnormalities in the signal-transduction pathways for granulocyte colony-stimulating factor (G-CSF) may play a part in the progression to acute myeloid leukemia.

Methods We isolated genomic DNA and RNA from hematopoietic cells obtained from two patients with acute myeloid leukemia and histories of severe congenital neutropenia. The nucleotide sequences encoding the cytoplasmic domain of the G-CSF receptor were amplified by means of the polymerase chain reaction and sequenced. Murine myeloid 32D.C10 cells were transfected with complementary DNA encoding the wild-type or mutant G-CSF receptors and tested for their responses to G-CSF.

Results Point mutations in the gene for the G-CSF receptor were identified in both patients. The mutations, a substitution of thymine for cytosine at the codon for glutamine at position 718 (Gln718) in one patient and at the codon for glutamine at position 731 (Gln731) in the other, caused a truncation of the C-terminal cytoplasmic region of the receptor. Both mutant and wild-type genes for the G-CSF receptor were present in leukemic cells from the two patients. In one patient, the mutation was also found in the neutropenic stage, before the progression to acute myeloid leukemia. The 32D.C10 cells expressing mutant receptors had abnormally high proliferative responses but failed to mature when cultured in G-CSF. The mutant G-CSF receptors also interfered with terminal maturation mediated by the wild-type G-CSF receptor in the 32D.C10 cells that coexpressed the wild-type and mutant receptors.

Conclusions Mutations in the gene for the G-CSF receptor that interrupt signals required for the maturation of myeloid cells are involved in the pathogenesis of severe congenital neutropenia and associated with the progression to acute myeloid leukemia.

Source Information

From the Department of Hematology, Dr. Daniel den Hoed Cancer Center and Institute of Hematology, Erasmus University, Rotterdam, the Netherlands (F.D., B.L., I.P.T.); the Department of Clinical Pathology, City of Hope National Medical Center, Duarte, Calif. (R.K.B.); and the Department of Pediatric Hematology and Oncology, Hannover Medical School, Hannover, Germany (N.T., K.W.).

Address reprint requests to Dr. Touw at the Dr. Daniel den Hoed Cancer Center, P.O. Box 5201, 3008 AE Rotterdam, the Netherlands.

Full Text of this Article

This article has been cited by other articles:

• Berliner, N. (2008). Lessons from congenital neutropenia: 50 years of progress in understanding myelopoiesis. Blood 111: 5427-5432 [Abstract] [Full Text]  
• Anderlini, P., Champlin, R. E. (2008). Biologic and molecular effects of granulocyte colony-stimulating factor in healthy individuals: recent findings and current challenges. Blood 111: 1767-1772 [Abstract] [Full Text]  
• Matsubara, K., Imai, K., Okada, S., Miki, M., Ishikawa, N., Tsumura, M., Kato, T., Ohara, O., Nonoyama, S., Kobayashi, M. (2007). Severe developmental delay and epilepsy in a Japanese patient with severe congenital neutropenia due to HAX1 deficiency. haematol 92: e123-e125 [Abstract] [Full Text]  
• Miranda, M. B., Redner, R. L., Johnson, D. E. (2007). Inhibition of Src family kinases enhances retinoic acid induced gene expression and myeloid differentiation. Molecular Cancer Therapeutics 6: 3081-3090 [Abstract] [Full Text]  
• Link, D. C., Kunter, G., Kasai, Y., Zhao, Y., Miner, T., McLellan, M. D., Ries, R. E., Kapur, D., Nagarajan, R., Dale, D. C., Bolyard, A. A., Boxer, L. A., Welte, K., Zeidler, C., Donadieu, J., Bellanne-Chantelot, C., Vardiman, J. W., Caligiuri, M. A., Bloomfield, C. D., DiPersio, J. F., Tomasson, M. H., Graubert, T. A., Westervelt, P., Watson, M., Shannon, W., Baty, J., Mardis, E. R., Wilson, R. K., Ley, T. J. (2007). Distinct patterns of mutations occurring in de novo AML versus AML arising in the setting of severe congenital neutropenia. Blood 110: 1648-1655 [Abstract] [Full Text]  
• Horwitz, M. S., Duan, Z., Korkmaz, B., Lee, H.-H., Mealiffe, M. E., Salipante, S. J. (2007). Neutrophil elastase in cyclic and severe congenital neutropenia. Blood 109: 1817-1824 [Abstract] [Full Text]  
• Touw, I. P., Bontenbal, M. (2007). Granulocyte Colony-Stimulating Factor: Key (F)actor or Innocent Bystander in the Development of Secondary Myeloid Malignancy?. JNCI J Natl Cancer Inst 99: 183-186 [Full Text]  
• Emanuel, P. D. (2007). Drifting precariously due to lost tyrosines. Blood 109: 7-8 [Full Text]  
• Germeshausen, M., Ballmaier, M., Welte, K. (2007). Incidence of CSF3R mutations in severe congenital neutropenia and relevance for leukemogenesis: results of a long-term survey. Blood 109: 93-99 [Abstract] [Full Text]  
• Mermel, C. H., McLemore, M. L., Liu, F., Pereira, S., Woloszynek, J., Lowell, C. A., Link, D. C. (2006). Src family kinases are important negative regulators of G-CSF-dependent granulopoiesis. Blood 108: 2562-2568 [Abstract] [Full Text]  
• Sloand, E. M., Yong, A. S. M., Ramkissoon, S., Solomou, E., Bruno, T. C., Kim, S., Fuhrer, M., Kajigaya, S., Barrett, A. J., Young, N. S. (2006). Granulocyte colony-stimulating factor preferentially stimulates proliferation of monosomy 7 cells bearing the isoform IV receptor. Proc. Natl. Acad. Sci. USA 103: 14483-14488 [Abstract] [Full Text]  
• Rosenberg, P. S., Alter, B. P., Bolyard, A. A., Bonilla, M. A., Boxer, L. A., Cham, B., Fier, C., Freedman, M., Kannourakis, G., Kinsey, S., Schwinzer, B., Zeidler, C., Welte, K., Dale, D. C., for the Severe Chronic Neutropenia International R, (2006). The incidence of leukemia and mortality from sepsis in patients with severe congenital neutropenia receiving long-term G-CSF therapy. Blood 107: 4628-4635 [Abstract] [Full Text]  
• Kaushansky, K. (2006). Lineage-specific hematopoietic growth factors.. NEJM 354: 2034-2045 [Full Text]  
• Zhuang, D., Qiu, Y., Kogan, S. C., Dong, F. (2006). Increased CCAAT Enhancer-binding Protein {epsilon} (C/EBP{epsilon}) Expression and Premature Apoptosis in Myeloid Cells Expressing Gfi-1 N382S Mutant Associated with Severe Congenital Neutropenia. J. Biol. Chem. 281: 10745-10751 [Abstract] [Full Text]  
• Liu, H., Qiu, Y., Xiao, L., Dong, F. (2006). Involvement of Protein Kinase C{epsilon} in the Negative Regulation of Akt Activation Stimulated by Granulocyte Colony-Stimulating Factor. J. Immunol. 176: 2407-2413 [Abstract] [Full Text]  
• Zhuang, D., Qiu, Y., Haque, S. J., Dong, F. (2005). Tyrosine 729 of the G-CSF receptor controls the duration of receptor signaling: involvement of SOCS3 and SOCS1. J. Leukoc. Biol. 78: 1008-1015 [Abstract] [Full Text]  
• Wolfler, A., Erkeland, S. J., Bodner, C., Valkhof, M., Renner, W., Leitner, C., Olipitz, W., Pfeilstocker, M., Tinchon, C., Emberger, W., Linkesch, W., Touw, I. P., Sill, H. (2005). A functional single-nucleotide polymorphism of the G-CSF receptor gene predisposes individuals to high-risk myelodysplastic syndrome. Blood 105: 3731-3736 [Abstract] [Full Text]  
• Druhan, L. J., Ai, J., Massullo, P., Kindwall-Keller, T., Ranalli, M. A., Avalos, B. R. (2005). Novel mechanism of G-CSF refractoriness in patients with severe congenital neutropenia. Blood 105: 584-591 [Abstract] [Full Text]  
• Andritsos, L. A., Adkins, D., Vij, R., DiPersio, J. F., Devine, S. M. (2004). Late Development of Acute Myeloid Leukemia in Two Peripheral Blood Stem Cell Donors Mobilized with G-CSF.. ASH ANNUAL MEETING ABSTRACTS 104: 1842-1842 [Abstract]  
• Hunter, M. G., Jacob, A., O'Donnell, L. C., Agler, A., Druhan, L. J., Coggeshall, K. M., Avalos, B. R. (2004). Loss of SHIP and CIS Recruitment to the Granulocyte Colony-Stimulating Factor Receptor Contribute to Hyperproliferative Responses in Severe Congenital Neutropenia/Acute Myelogenous Leukemia. J. Immunol. 173: 5036-5045 [Abstract] [Full Text]  
• van de Geijn, G.-J. M., Gits, J., Aarts, L. H. J., Heijmans-Antonissen, C., Touw, I. P. (2004). G-CSF receptor truncations found in SCN/AML relieve SOCS3-controlled inhibition of STAT5 but leave suppression of STAT3 intact. Blood 104: 667-674 [Abstract] [Full Text]  
• Kuramoto, K., Follmann, D. A., Hematti, P., Sellers, S., Agricola, B. A., Metzger, M. E., Donahue, R. E., von Kalle, C., Dunbar, C. E. (2004). Effect of chronic cytokine therapy on clonal dynamics in nonhuman primates. Blood 103: 4070-4077 [Abstract] [Full Text]  
• Aarts, L. H. J., Roovers, O., Ward, A. C., Touw, I. P. (2004). Receptor activation and 2 distinct COOH-terminal motifs control G-CSF receptor distribution and internalization kinetics. Blood 103: 571-579 [Abstract] [Full Text]  
• Relling, M. V., Boyett, J. M., Blanco, J. G., Raimondi, S., Behm, F. G., Sandlund, J. T., Rivera, G. K., Kun, L. E., Evans, W. E., Pui, C.-H. (2003). Granulocyte colony-stimulating factor and the risk of secondary myeloid malignancy after etoposide treatment. Blood 101: 3862-3867 [Abstract] [Full Text]  
• Hermans, M. H. A., van de Geijn, G.-J., Antonissen, C., Gits, J., van Leeuwen, D., Ward, A. C., Touw, I. P. (2003). Signaling mechanisms coupled to tyrosines in the granulocyte colony-stimulating factor receptor orchestrate G-CSF-induced expansion of myeloid progenitor cells. Blood 101: 2584-2590 [Abstract] [Full Text]  
• Grenda, D. S., Johnson, S. E., Mayer, J. R., McLemore, M. L., Benson, K. F., Horwitz, M., Link, D. C. (2002). Mice expressing a neutrophil elastase mutation derived from patients with severe congenital neutropenia have normal granulopoiesis. Blood 100: 3221-3228 [Abstract] [Full Text]  
• Hortner, M., Nielsch, U., Mayr, L. M., Johnston, J. A., Heinrich, P. C., Haan, S. (2002). Suppressor of Cytokine Signaling-3 Is Recruited to the Activated Granulocyte-Colony Stimulating Factor Receptor and Modulates its Signal Transduction. J. Immunol. 169: 1219-1227 [Abstract] [Full Text]  
• Dong, F., Qiu, Y., Yi, T., Touw, I. P., Larner, A. C. (2001). The Carboxyl Terminus of the Granulocyte Colony- Stimulating Factor Receptor, Truncated in Patients with Severe Congenital Neutropenia/Acute Myeloid Leukemia, Is Required for SH2-Containing Phosphatase-1 Suppression of Stat Activation. J. Immunol. 167: 6447-6452 [Abstract] [Full Text]  
• Magnusson, M. K., Meade, K. E., Brown, K. E., Arthur, D. C., Krueger, L. A., Barrett, A. J., Dunbar, C. E. (2001). Rabaptin-5 is a novel fusion partner to platelet-derived growth factor {beta} receptor in chronic myelomonocytic leukemia. Blood 98: 2518-2525 [Abstract] [Full Text]  
• Hermans, M. H. A., Touw, I. P., Dale, D. C., Aprikyan, A. (2001). Significance of neutrophil elastase mutations versus G-CSF receptor mutations for leukemic progression of congenital neutropenia. Blood 97: 2185-2186 [Full Text]  
• Tschan, C. A., Pilz, C., Zeidler, C., Welte, K., Germeshausen, M. (2001). Time course of increasing numbers of mutations in the granulocyte colony-stimulating factor receptor gene in a patient with congenital neutropenia who developed leukemia. Blood 97: 1882-1884 [Abstract] [Full Text]  
• Lakshman, R, Finn, A (2001). Neutrophil disorders and their management. J. Clin. Pathol. 54: 7-19 [Abstract] [Full Text]  
• Jeha, S., Chan, K. W., Aprikyan, A. G., Hoots, W. K., Culbert, S., Zietz, H., Dale, D. C., Albitar, M. (2000). Spontaneous remission of granulocyte colony-stimulating factor-associated leukemia in a child with severe congenital neutropenia. Blood 96: 3647-3649 [Abstract] [Full Text]  
• Dale, D. C., Person, R. E., Bolyard, A. A., Aprikyan, A. G., Bos, C., Bonilla, M. A., Boxer, L. A., Kannourakis, G., Zeidler, C., Welte, K., Benson, K. F., Horwitz, M. (2000). Mutations in the gene encoding neutrophil elastase in congenital and cyclic neutropenia. Blood 96: 2317-2322 [Abstract] [Full Text]  
• Modi, N., Carr, R. (2000). Promising stratagems for reducing the burden of neonatal sepsis. Arch. Dis. Child. Fetal Neonatal Ed. 83: 150F-153 [Full Text]  
• Katsuki, K., Shinohara, K., Takeda, K., Ariyoshi, K., Yamada, T., Kameda, N., Takahashi, T., Nawata, R., Shibata, S., Asano, Y., Okamura, S. (2000). Chronic Neutrophilic Leukemia with Acute Myeloblastic Transformation. Jpn J Clin Oncol 30: 362-365 [Abstract] [Full Text]  
• Freedman, M. H., Bonilla, M. A., Fier, C., Bolyard, A. A., Scarlata, D., Boxer, L. A., Brown, S., Cham, B., Kannourakis, G., Kinsey, S. E., Mori, P. G., Cottle, T., Welte, K., Dale, D. C. (2000). Myelodysplasia syndrome and acute myeloid leukemia in patients with congenital neutropenia receiving G-CSF therapy. Blood 96: 429-436 [Abstract] [Full Text]  
• Sawai, N., Koike, K., Mwamtemi, H. H., Ito, S., Kurokawa, Y., Sakashita, K., Kinoshita, T., Higuchi, T., Takeuchi, K., Shiohara, M., Kamijo, T., Higuchi, Y., Miyazaki, H., Kato, T., Kobayashi, M., Miyake, M., Yasui, K., Komiyama, A. (2000). Thrombopoietin enhances neutrophil production by bone marrow hematopoietic progenitors with the aid of stem cell factor in congenital neutropenia. J. Leukoc. Biol. 68: 137-143 [Abstract] [Full Text]  
• Shimizu, K., Kitabayashi, I., Kamada, N., Abe, T., Maseki, N., Suzukawa, K., Ohki, M. (2000). AML1-MTG8 leukemic protein induces the expression of granulocyte colony-stimulating factor (G-CSF) receptor through the up-regulation of CCAAT/enhancer binding protein epsilon. Blood 96: 288-296 [Abstract] [Full Text]  
• White, S. M., Alarcon, M. H., Tweardy, D. J. (2000). Inhibition of granulocyte colony-stimulating factor-mediated myeloid maturation by low level expression of the differentiation-defective class IV granulocyte colony-stimulating factor receptor isoform. Blood 95: 3335-3340 [Abstract] [Full Text]  
• Fujio, K., Nosaka, T., Kojima, T., Kawashima, T., Yahata, T., Copeland, N. G., Gilbert, D. J., Jenkins, N. A., Yamamoto, K., Nishimura, T., Kitamura, T. (2000). Molecular cloning of a novel type 1 cytokine receptor similar to the common gamma chain. Blood 95: 2204-2210 [Abstract] [Full Text]  
• Hunter, M. G., Avalos, B. R. (2000). Granulocyte colony-stimulating factor receptor mutations in severe congenital neutropenia transforming to acute myelogenous leukemia confer resistance to apoptosis and enhance cell survival. Blood 95: 2132-2137 [Abstract] [Full Text]  
• Dong, F., Larner, A. C. (2000). Activation of Akt kinase by granulocyte colony-stimulating factor (G-CSF): evidence for the role of a tyrosine kinase activity distinct from the janus kinases. Blood 95: 1656-1662 [Abstract] [Full Text]  
• Zeidler, C., Welte, K., Barak, Y., Barriga, F., Bolyard, A. A., Boxer, L., Cornu, G., Cowan, M. J., Dale, D. C., Flood, T., Freedman, M., Gadner, H., Mandel, H., O'Reilly, R. J., Ramenghi, U., Reiter, A., Skinner, R., Vermylen, C., Levine, J. E. (2000). Stem cell transplantation in patients with severe congenital neutropenia without evidence of leukemic transformation. Blood 95: 1195-1198 [Abstract] [Full Text]  
• Dinauer, M. C., Lekstrom-Himes, J. A., Dale, D. C. (2000). Inherited Neutrophil Disorders: Molecular Basis and New Therapies. ASH Education Book 2000: 303-318 [Abstract] [Full Text]  
• Ward, A. C., Touw, I., Yoshimura, A. (2000). The Jak-Stat pathway in normal and perturbed hematopoiesis. Blood 95: 19-29 [Full Text]  
• Konishi, N., Kobayashi, M., Miyagawa, S.-i., Sato, T., Katoh, O., Ueda, K. (1999). Defective Proliferation of Primitive Myeloid Progenitor Cells in Patients With Severe Congenital Neutropenia. Blood 94: 4077-4083 [Abstract] [Full Text]  
• Moliterno, A. R., Spivak, J. L. (1999). Posttranslational Processing of the Thrombopoietin Receptor Is Impaired in Polycythemia Vera. Blood 94: 2555-2561 [Abstract] [Full Text]  
• Lowenberg, B., Downing, J. R., Burnett, A. (1999). Acute Myeloid Leukemia. NEJM 341: 1051-1062 [Full Text]  
• Ward, A. C., van Aesch, Y. M., Gits, J., Schelen, A. M., de Koning, J. P., van Leeuwen, D., Freedman, M. H., Touw, I. P. (1999). Novel Point Mutation in the Extracellular Domain of the Granulocyte Colony-stimulating Factor (G-CSF) Receptor in a Case of Severe Congenital Neutropenia Hyporesponsive to G-CSF Treatment. J. Exp. Med. 190: 497-508 [Abstract] [Full Text]  
• Bogardus, S. T. Jr, Concato, J., Feinstein, A. R. (1999). Clinical Epidemiological Quality in Molecular Genetic Research: The Need for Methodological Standards. JAMA 281: 1919-1926 [Abstract] [Full Text]  
• Ward, A. C., Smith, L., de Koning, J. P., van Aesch, Y., Touw, I. P. (1999). Multiple Signals Mediate Proliferation, Differentiation, and Survival from the Granulocyte Colony-stimulating Factor Receptor in Myeloid 32D Cells. J. Biol. Chem. 274: 14956-14962 [Abstract] [Full Text]  
• Carr, R., Modi, N., Doré, C. J., El-Rifai, R., Lindo, D. (1999). A Randomized, Controlled Trial of Prophylactic Granulocyte-Macrophage Colony-Stimulating Factor in Human Newborns Less Than 32 Weeks Gestation. Pediatrics 103: 796-802 [Abstract] [Full Text]  
• Hermans, M. H.A., Antonissen, C., Ward, A. C., Mayen, A. E.M., Ploemacher, R. E., Touw, I. P. (1999). Sustained Receptor Activation and Hyperproliferation in Response to Granulocyte Colony-stimulating Factor (G-CSF) in Mice with a Severe Congenital Neutropenia/Acute Myeloid Leukemia-derived Mutation in the G-CSF Receptor Gene. J. Exp. Med. 189: 683-692 [Abstract] [Full Text]  
• Hunter, M. G., Avalos, B. R. (1999). Deletion of a Critical Internalization Domain in the G-CSFR in Acute Myelogenous Leukemia Preceded by Severe Congenital Neutropenia. Blood 93: 440-446 [Abstract] [Full Text]  
• Ward, A. C., van Aesch, Y. M., Schelen, A. M., Touw, I. P. (1999). Defective Internalization and Sustained Activation of Truncated Granulocyte Colony-Stimulating Factor Receptor Found in Severe Congenital Neutropenia/Acute Myeloid Leukemia. Blood 93: 447-458 [Abstract] [Full Text]  
• Ward, A. C., Hermans, M. H.A., Smith, L., van Aesch, Y. M., Schelen, A. M., Antonissen, C., Touw, I. P. (1999). Tyrosine-Dependent and -Independent Mechanisms of STAT3 Activation by the Human Granulocyte Colony-Stimulating Factor (G-CSF) Receptor Are Differentially Utilized Depending on G-CSF Concentration. Blood 93: 113-124 [Abstract] [Full Text]  
• Dong, F., Liu, X., de Koning, J. P., Touw, I. P., Henninghausen, L., Larner, A., Grimley, P. M. (1998). Stimulation of Stat5 by Granulocyte Colony-Stimulating Factor (G-CSF) Is Modulated by Two Distinct Cytoplasmic Regions of the G-CSF Receptor. J. Immunol. 161: 6503-6509 [Abstract] [Full Text]  
• Solder, B., Weiss, M., Jager, A., Belohradsky, B. H. (1998). Dyskeratosis Congenita: Multisystemic Disorder with Special Consideration of Immunologic Aspects: A Review of the Literature. CLIN PEDIATR 37: 521-530 [Abstract]  
• Harris, K. W., Hu, X.-J., Schultz, S., Arcasoy, M. O., Forget, B. G., Clare, N. (1998). The Distal Cytoplasmic Domain of the Erythropoietin Receptor Induces Granulocytic Differentiation in 32D Cells. Blood 92: 1219-1224 [Abstract] [Full Text]  
• Jacob, J., Haug, J. S., Raptis, S., Link, D. C. (1998). Specific Signals Generated by the Cytoplasmic Domain of the Granulocyte Colony-Stimulating Factor (G-CSF) Receptor Are Not Required for G-CSF-Dependent Granulocytic Differentiation. Blood 92: 353-361 [Abstract] [Full Text]  
• Hermans, M. H.A., Ward, A. C., Antonissen, C., Karis, A., Lowenberg, B., Touw, I. P. (1998). Perturbed Granulopoiesis in Mice With a Targeted Mutation in the Granulocyte Colony-Stimulating Factor Receptor Gene Associated With Severe Chronic Neutropenia. Blood 92: 32-39 [Abstract] [Full Text]  
• Socolovsky, M., Lodish, H. F., Daley, G. Q. (1998). Control of hematopoietic differentiation: Lack of specificity in signaling by cytokine receptors. Proc. Natl. Acad. Sci. USA 95: 6573-6575 [Full Text]  
• Hunter, M. G., Avalos, B. R. (1998). Phosphatidylinositol 3'-Kinase and SH2-Containing Inositol Phosphatase (SHIP) Are Recruited by Distinct Positive and Negative Growth-Regulatory Domains in the Granulocyte Colony-Stimulating Factor Receptor. J. Immunol. 160: 4979-4987 [Abstract] [Full Text]  
• de Koning, J. P., Soede-Bobok, A. A., Schelen, A. M., Smith, L., van Leeuwen, D., Santini, V., Burgering, B. M.T., Bos, J. L., Lowenberg, B., Touw, I. P. (1998). Proliferation Signaling and Activation of Shc, p21Ras, and Myc Via Tyrosine 764 of Human Granulocyte Colony-Stimulating Factor Receptor. Blood 91: 1924-1933 [Abstract] [Full Text]  
• Gale, R. E., Freeburn, R. W., Khwaja, A., Chopra, R., Linch, D. C. (1998). A Truncated Isoform of the Human beta  Chain Common to the Receptors for Granulocyte-Macrophage Colony-Stimulating Factor, Interleukin-3 (IL-3), and IL-5 With Increased mRNA Expression in Some Patients With Acute Leukemia. Blood 91: 54-63 [Abstract] [Full Text]  
• Kasper, B., Herbst, A., Pilz, C., Germeshausen, M., Tidow, N., Hadam, M. R., Welte, K. (1997). Severe Congenital Neutropenia Patients With Point Mutations in the Granulocyte Colony-Stimulating Factor (G-CSF ) Receptor mRNA Express a Normal G-CSF Receptor Protein. Blood 90: 2839-2840 [Full Text]  
• Pui, C.-H., Boyett, J. M., Hughes, W. T., Rivera, G. K., Hancock, M. L., Sandlund, J. T., Synold, T., Relling, M. V., Ribeiro, R. C., Crist, W. M., Evans, W. E. (1997). Human Granulocyte Colony-Stimulating Factor after Induction Chemotherapy in Children with Acute Lymphoblastic Leukemia. NEJM 336: 1781-1787 [Abstract] [Full Text]  
• Tidow, N., Pilz, C., Teichmann, B., Muller-Brechlin, A., Germeshausen, M., Kasper, B., Rauprich, P., Sykora, K.-W., Welte, K. (1997). Clinical Relevance of Point Mutations in the Cytoplasmic Domain of the Granulocyte Colony-Stimulating Factor Receptor Gene in Patients With Severe Congenital Neutropenia. Blood 89: 2369-2375 [Abstract] [Full Text]  
• Carr, R., Modi, N. (1997). Haemopoietic colony stimulating factors for preterm neonates. Arch. Dis. Child. Fetal Neonatal Ed. 76: 128F-133 [Full Text]  
• Calhoun, D. A., Christensen, R. D. (1997). The Occurrence of Kostmann Syndrome in Preterm Neonates. Pediatrics 99: 259-259 [Full Text]  
• Alter, B. P. (1996). Aplastic Anemia, Pediatric Aspects. The Oncologist 1: 361-366 [Abstract] [Full Text]  
• Naparstek, E. (1995). Granulocyte Colony-Stimulating Factor, Congenital Neutropenia, and Acute Myeloid Leukemia. NEJM 333: 516-518 [Full Text]  
• Dong, F., Gutkind, J. S., Larner, A. C. (2001). Granulocyte Colony-stimulating Factor Induces Erk5 Activation, Which Is Differentially Regulated by Protein-tyrosine Kinases and Protein Kinase C. REGULATION OF CELL PROLIFERATION AND SURVIVAL. J. Biol. Chem. 276: 10811-10816 [Abstract] [Full Text]  
• Li, F.-Q., Horwitz, M. (2001). Characterization of Mutant Neutrophil Elastase in Severe Congenital Neutropenia. J. Biol. Chem. 276: 14230-14241 [Abstract] [Full Text]  
• Kroll, S. L., Barth-Baus, D., Hensold, J. O. (2001). The Carboxyl-terminal Domain of the Granulocyte Colony-stimulating Factor Receptor Uncouples Ribosomal Biogenesis from Cell Cycle Progression in Differentiating 32D Myeloid Cells. J. Biol. Chem. 276: 49410-49418 [Abstract] [Full Text]