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Previous Volume 345:1388-1393 November 8, 2001 Number 19 Next

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ABSTRACT

Background Although semen analysis is routinely used to evaluate the male partner in infertile couples, sperm measurements that discriminate between fertile and infertile men are not well defined.

Methods We evaluated two semen specimens from each of the male partners in 765 infertile couples and 696 fertile couples at nine sites. The female partners in the infertile couples had normal results on fertility evaluation. The sperm concentration and motility were determined at the sites; semen smears were stained at the sites and shipped to a central laboratory for an assessment of morphologic features of sperm with the use of strict criteria. We used classification-and-regression-tree analysis to estimate threshold values for subfertility and fertility with respect to the sperm concentration, motility, and morphology. We also used an analysis of receiver-operating-characteristic curves to assess the relative value of these sperm measurements in discriminating between fertile and infertile men.

Results The subfertile ranges were a sperm concentration of less than 13.5x10⁶ per milliliter, less than 32 percent of sperm with motility, and less than 9 percent with normal morphologic features. The fertile ranges were a concentration of more than 48.0x10⁶ per milliliter, greater than 63 percent motility, and greater than 12 percent normal morphologic features. Values between these ranges indicated indeterminate fertility. There was extensive overlap between the fertile and the infertile men within both the subfertile and the fertile ranges for all three measurements. Although each of the sperm measurements helped to distinguish between fertile and infertile men, none was a powerful discriminator. The percentage of sperm with normal morphologic features had the greatest discriminatory power.

Conclusions Threshold values for sperm concentration, motility, and morphology can be used to classify men as subfertile, of indeterminate fertility, or fertile. None of the measures, however, are diagnostic of infertility.

Source Information

From the University of Rochester, Rochester, N.Y. (D.S.G.); the University of California, Davis (J.W.O., C.K.B., S.T.N.); Columbia University, New York (P.F.-L., D.X.); the University of Pennsylvania Medical Center, Philadelphia (C.C.); Baylor College of Medicine, Houston (S.A.C., P.C.); the University of Alabama, Birmingham (M.P.S.); Brigham and Women's Hospital, Boston (J.A.H.); and the National Institutes of Health, Bethesda, Md. (D.L.V.).

Address reprint requests to Dr. Guzick at the Department of Obstetrics and Gynecology, University of Rochester School of Medicine and Dentistry, Box 668, 601 Elmwood Ave., Rochester, NY 14642, or at david_guzick{at}urmc.rochester.edu.

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