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Correspondence
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Volume 350:1577-1579 April 8, 2004 Number 15
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Boosting the Sensitivity of Real-Time Polymerase-Chain-Reaction Testing for SARS

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To the Editor: In view of recent concern about the recurrence of severe acute respiratory syndrome (SARS) in Guangdong, China, we would like to highlight the tremendous importance of sensitivity in testing for the SARS-associated coronavirus (SARS-CoV). Because the initial symptoms of SARS are similar to those of other common respiratory diseases, making a specific diagnosis of SARS poses difficulties to medical professionals. Our enhanced real-time (ERT) polymerase-chain-reaction (PCR) method (first presented in June 2003 at a symposium on SARS1) has been designed for the detection of SARS-CoV with high sensitivity and easy-to-interpret results.2 The power of the ERT . . . [Full Text of this Article]


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