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Previous Volume 356:11-20 January 4, 2007 Number 1 Next

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ABSTRACT

Background Current staging methods are inadequate for predicting the outcome of treatment of non–small-cell lung cancer (NSCLC). We developed a five-gene signature that is closely associated with survival of patients with NSCLC.

Methods We used computer-generated random numbers to assign 185 frozen specimens for microarray analysis, real-time reverse-transcriptase polymerase chain reaction (RT-PCR) analysis, or both. We studied gene expression in frozen specimens of lung-cancer tissue from 125 randomly selected patients who had undergone surgical resection of NSCLC and evaluated the association between the level of expression and survival. We used risk scores and decision-tree analysis to develop a gene-expression model for the prediction of the outcome of treatment of NSCLC. For validation, we used randomly assigned specimens from 60 other patients.

Results Sixteen genes that correlated with survival among patients with NSCLC were identified by analyzing microarray data and risk scores. We selected five genes (DUSP6, MMD, STAT1, ERBB3, and LCK) for RT-PCR and decision-tree analysis. The five-gene signature was an independent predictor of relapse-free and overall survival. We validated the model with data from an independent cohort of 60 patients with NSCLC and with a set of published microarray data from 86 patients with NSCLC.

Conclusions Our five-gene signature is closely associated with relapse-free and overall survival among patients with NSCLC.

Source Information

From National Taiwan University College of Public Health (H.-Y.C., W.J.C.), National Taiwan University College of Medicine (H.-Y.C., S.-L.Y., C.-L.C., C.-H.W., S.-F.K., H.-N.L., S.S., W.J.C., J.J.W.C., P.-C.Y.), Academia Sinica (C.-H.C, P.-C.Y.), National Taiwan University Hospital (A.Y., W.-K.C., P.-C.Y.), and Advpharma (H.-J.T.) — all in Taipei, Taiwan; and Taichung Veterans General Hospital (G.-C.C., C.-Y.C.) and National Chung-Hsing University (G.-C.C., C.-C.L., J.J.W.C.) — both in Taichung, Taiwan.

Drs. W.J. Chen, J.J.W. Chen, and P.C. Yang contributed equally to this article.

Address reprint requests to Dr. Yang at the Department of Internal Medicine, National Taiwan University Hospital, No. 7, Chung-Shan S. Rd., Taipei, Taiwan 100, or at pcyang{at}ha.mc.ntu.edu.tw.

Full Text of this Article

Related Letters:

Five-Gene Signature in Non–Small-Cell Lung Cancer
Michiels S., Hill C., Raz D. J., Jablons D. M., Dobbin K. K., Gounaris I., Quintás-Cardama A., Gibbons D. L., Chen H.-Y., Chen W. J., Yang P.-C.
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N Engl J Med 2007; 356:1581-1583, Apr 12, 2007. Correspondence

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